alexa Glycomic Signature of Mouse Embryonic Stem Cells During Differentiation | OMICS International
ISSN: 2168-9296

Cell & Developmental Biology
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Research Article

Glycomic Signature of Mouse Embryonic Stem Cells During Differentiation

Rania Harfouche1,2*, Somak Ray3, Melinda Sanchez1, Ushashi Dadwal1,2, Steven R Head4, Aaron Goldman1,2 and Shiladitya Sengupta1,2,5,6*
1BWH-HST Center for Biomedical Engineering, Harvard Medical School, Cambridge, USA
2Harvard-MIT Division of Health Sciences and Technology, Harvard Medical School, Cambridge, USA
3The Barnett Institute, Boston, USA
4DNA Array Core Facility, The Scripps Research Institute, La Jolla, USA
5Channing Laboratory, Department of Medicine, Brigham and Women’s Hospital, Harvard Medical School, Cambridge, USA
6Dana Farber Cancer Institute, Boston, USA
Corresponding Authors : Rania Harfouche
BWH-HST Center for Biomedical Engineering
Harvard Medical School
65 Landsdowne Street, Cambridge
MA 02139, USA
E-mail: [email protected]
  Shiladitya Sengupta
BWH-HST Center for Biomedical Engineering
Harvard Medical School
65 Landsdowne Street Cambridge
MA 02139, USA
Received July 22, 2013; Accepted August 21, 2013; Published August 24, 2013
Citation: Harfouche R, Ray S, Sanchez M, Dadwal U, Head SR, et al. (2013) Glycomic Signature of Mouse Embryonic Stem Cells During Differentiation. Cell Dev Biol 2:120. doi:10.4172/2168-9296.1000120
Copyright: © 2013 Harfouche R, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: The glycome has emerged as a key regulator of cell fate, partly through its ability to potentiate the action of numerous signaling pathways. We recently demonstrated that a sulfated component of the glycome plays a critical role in promoting the differentiation of embryonic stem cell (ESC)-derived embryoid bodies by modulating downstream growth factors, such as the insulin-like growth factor (IGF) signaling axis. However, the exact components of the glycome which promote ESC differentiation versus stemness remain uncharacterized, due to the lack of a rapid, simple and easily quantifiable methodology. As a proof-of-concept in this study, we utilized a custom-made glycoarray in combination with bioinformatics and molecular biology tools in order to uncover novel glyco-signatures underlying ESC differention in an embryoid body model. A better elucidation of the glycomic transcriptomal signature underlying ESC differentiation would allow us to better manipulate these cells towards a desired lineage.

Method: We used a custom-designed Affymetrix microarray, the Glycogene-chip, to screen the transcriptome of differentiating embryoid bodies versus that of undifferentiated ESC. In conjunction with gene ontology, pathway analyses, real-time PCR and immunoblotting, we validated the involvement of the IGF family, and furthermore, uncovered novel differentially regulated genes belonging to the glycoprotein (Angiopoietin-1 and Angiopoietin-like members), sulfotransferase, sulfatase and glycosyltransferase families.

Conclusion: These results suggest that the Glycogene-chip, in conjunction with the embryoid body model, provides a fast and reliable tool to uncover novel glycomic signatures that are critical to maitain ESC stemness versus differentiation. In turn, this will allow us to understand the mechanisms governing ESC fate, bringing us one step closer towards finding a new paradigm for the regenerative medicine field.

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