Glycoproteomic Analysis of Human Hepatoblastoma Cell Lines Using Glycopeptide Capture and Mass SpectrometryQinzhe Wang†, Fei Zhao†, Xiuxuan Sun, Shisheng Sun, Hanjie Yu, Yaogang Zhong, Yuan Hu, Zhuo Chen, Peixin Zhang and Zheng Li*
Laboratory for Functional Glycomics, College of Life Sciences, Northwest University, Xi’an 710069, China
†These authors contributed equally to this work
- *Corresponding Author:
- Zheng Li
Laboratory for Functional Glycomics
College of Life Sciences, Northwest University
229 Taibai Beilu, Xi’an 710069, P. R. China
E-mail: [email protected]
Received date: June 11, 2015 Accepted date: July 03, 2015 Published date: July 15, 2015
Citation: Wang Q, Zhao F, Sun X, Sun S, Yu H, et al. (2015) Glycoproteomic Analysis of Human Hepatoblastoma Cell Lines Using Glycopeptide Capture and Mass Spectrometry. J Glycomics Lipidomics 5:132. doi:10.4172/2153-0637.1000132
Copyright: © 2015 Wang Q, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Hepatoblastoma (HB) is the most common form of liver tumour in infants and children. The cancer cell line HepG2 and normal hepatocellular cell line L02 are valuable cell models and are already widely used in the world while their glycopeoteome profiles are still unknown. This study focuses on N-linked glycoproteomic analysis of these two cell lines using mass spectrometry. Using two complementary approaches, Hydrazide reaction and hydrophilic affinity solid phase extraction methods, almost 400 glycosylation sites were identified from the two cell lines. Functional annotation suggests that N-glycoproteins with molecular binding ability were enriched in HepG2 cells compared to normal liver cells.