Graft Composition and Post-Thawing Cell Viability Influence the Hematopoietic Recovery in Autologous Hematopoietic Stem Cell TransplantationCaterina Giovanna Valentini, Maria Bianchi, Nicoletta Orlando, Francesco Autore, Maria Grazia Iachininoto, Nicola Piccirillo, Simona Sica, Gina Zini, Valerio De Stefano and Luciana Teofili*
Institute of Hematology, Catholic University, Rome, Italy
- *Corresponding Author:
- Luciana Teofili, MD
Institute of Hematology
Catholic University, Largo Gemelli
8I-00168 Rome, Italy
E-mail: [email protected]
Received date: February 24, 2017; Accepted date: March 13, 2017; Published date: March 20, 2017
Citation: Valentini CG, Bianchi M, Orlando N, Autore F, Iachininoto MG, et al. (2017) Graft Composition and Post-Thawing Cell Viability Influence the Hematopoietic Recovery in Autologous Hematopoietic Stem Cell Transplantation. J Stem Cell Res Ther 7:379. doi: 10.4172/2157-7633.1000379
Copyright: © 2017 Valentini CG, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: We investigated the influence of graft composition and post-thawing cell viability on the hematopoietic recovery of patients undergoing Autologous Hematopoietic Stem Cell Transplantation (ASCT).
Methods: Data relative to 146 ASCT procedures performed in 134 patients were examined. The doses of CD34+ cells and parameters related to the grafts’ composition (white blood cell – WBC, neutrophil and platelet -PLT concentrations) were correlated to the number of days for neutrophil and platelet engraftment. Moreover, patients were grouped according to the values of post-thawing total nucleated cell (TNC) and CD34+ cell viability (as lower of higher than median values of the entire series) and hematopoietic recovery was accordingly evaluated.
Results: The CD34+ cell dose significantly predicts both neutrophil and PLT engraftment. Patients with low TNC viability had longer time to neutrophil engraftment, while patients with low CD34+ cell viability exhibited longer time to both neutrophil and PLT engraftment. High WBC or neutrophil concentrations in the graft were associated with low TNC viability. In addition, we found an inverse correlation between CD34+ cell viability and graft PLT concentration.
Conclusions: Our findings suggest that the platelet content in apheresis products is a critical issue, with an impact on CD34+ cell viability and hematopoietic recovery after ASCT. The apheresis products containing high amounts of PLT should be evaluated for PLT removal before freezing.