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Graphene Oxide based Label Free Ultrasensitive Immunosensor for Lung Cancer Biomarker, hTERT | OMICS International | Abstract
ISSN: 2155-6210

Journal of Biosensors & Bioelectronics
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Research Article

Graphene Oxide based Label Free Ultrasensitive Immunosensor for Lung Cancer Biomarker, hTERT

Meenakshi Choudhary1,2, Veeresh Kumar3,4, Anu Singh1, Manoj P. Singh1, Satbir Kaur2, G.B. Reddy4, Renu Pasricha3, Surinder P. Singh3 and Kavita Arora1*

1Advanced Instrumentation Research Facility (AIRF), Jawaharlal Nehru University (JNU), New Mehrauli Road, New Delhi, India

2Department of Human Genetics, Punjabi University, Patiala, Punjab, India

3CSIR-National Physical Laboratory (NPL), Dr. K. S. Krishnan Marg, New Delhi, India

4Department of Physics, Indian Institute of Technology, Hauz Khas, New Delhi, India

*Corresponding Author:
Kavita Arora
Advanced Instrumentation Research Facility (AIRF)
Jawaharlal Nehru University (JNU)
New Mehrauli Road, New Delhi, India
E-mail: [email protected]

Received Date: July 30, 2013; Accepted Date: September 06, 2013; Published Date: September 13, 2013

Citation: Choudhary M, Kumar V, Singh A, Kaur S, Reddy GB, et al. (2013) Graphene Oxide based Label Free Ultrasensitive Immunosensor for Lung Cancer Biomarker, hTERT. J Biosens Bioelectron 4:143. doi: 10.4172/2155-6210.1000143

Copyright: © 2013 Choudhary M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

We report the fabrication of ultrasensitive Graphene Oxide (GO) based electrochemical immunosensor to detect human telomerase reverse transcriptase (hTERT), a lung cancer biomarker. The immuno-electrode-has been fabricated by covalent immobilization of rabbit anti-hTERT antibodies (Ab) onto GO films on ITO coated glass. The Fourier Transform Infrared (FTIR) spectroscopic studies confirms the presence of diverse organic functional groups (-COOH, -CHO, -OH) of GO, and the binding (anti-hTERT) onto GO/ITO electrode. Interestingly, Scanning Electron Micrographs (SEM) also reveals clear visual surface modification of the GO film by anti-hTERT antibodies and hTERT antigen (Ag). The electrochemical Differential Pulse Voltammetry (DPV) results show that the GO based immunosensor exhibits specificity and low detection upto 10 ag mL-1 (10×10-18 g mL-1) in wide detection range (10 ag mL-1-50 ng mL-1) for hTERT. The immunosensor showed ability to detect hTERT in spiked sputum samples upto 100 fg mL-1 in dynamic detection range of 100 fg mL-1-10 ng mL-1. The enhanced performance of Ab/GO/ITO is attributed to fast electron transfer and efficient loading of Ab on large surface area provided by GO network. The low level detection of hTERT warrants the realization of point-of-care device for early detection of lung/oral cancer through oral fluids

Keywords

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