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ISSN: 2157-7439

Journal of Nanomedicine & Nanotechnology
Open Access

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Research Article

Green Synthesis of Copper-Chitosan Nanoparticles and Study of its Antibacterial Activity

Appu Manikandan and Muthukrishnan Sathiyabama*

Department of Plant Science, Bharathidasan University, Tiruchirappalli, Tamil Nadu, India

*Corresponding Author:
Muthukrishnan Sathiyabam
Department of Plant Science
Bharathidasan University
Tiruchirappalli, Tamil Nadu, India
E-mail: [email protected]

Received Date: October 21, 2014; Accepted Date: December 06, 2014; Published Date: January 01, 2015

Citation: Manikandan A, Sathiyabama M (2015) Green Synthesis of Copper-Chitosan Nanoparticles and Study of its Antibacterial Activity. J Nanomed Nanotechnol 5:251. doi:10.4172/2157-7439.1000251

Copyright: © 2015 Manikandan A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Copper nanoparticle (Cu-NP) synthesis has been gaining attention due to its property and applicability. The main objective of the work is to synthesize stable Cu-NP through green synthesis. This work demonstrates the synthesis of Cu-NP by the addition of the acidic chitosan solution to CuSO4 solution with constant stirring at 70°C for 12 h. The addition of chitosan aids the stable formation of nanoparticle. The formation of nanoparticle was observed by the peak at 536 nm using UV-Vis spectroscopy. The interaction between the chitosan and the synthesized nanoparticles was studied using FTIR spectroscopy. The average size of the nanoparticle as determined by zetasizer was about 88.21 nm, while the zeta potential was -29.0 mV. Transmission electron microscopy (TEM) analysis indicated the spherical size with a size ranging between 20-30 nm. The Energy-dispersive X-ray spectroscopy (EDS) analysis showed a homogenous copper-rich composition of metal nanoparticles. The X-ray diffraction (XRD) analysis confirmed the presence of copper nanoparticle with crystalline nature. The synthesised Cu-chitosan nanoparticle exhibited antibacterial activity against gram negative as well as gram positive bacteria. However, the activity was more against gram negative bacteria which may be due to the difference in cell wall composition.

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