alexa Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation
ISSN: 2161-0940

Anatomy & Physiology: Current Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation

Larguech G1, Cesaro A1, Toumi H1,2*, Martinić I3, Petoud S3, Daniellou R4 and Lespessailles E1,2

1University of Orléans, I3MTO, EA 4708, F-45032 Orléans, France

2Service of Rhumatology, Center Regional Hospital of Orléans, 14 Avenue of the Hospital, Orléans- 45100, France

3Center of Molecular Biophysics, CNRS UPR 4301, Orléans- 45071, France

4University of Orléans, CNRS, ICOA, UMR 7311, F-45067 Orléans, France

*Corresponding Author:
Toumi H
Service of Rhumatology, Center Regional Hospital of Orléans
14 Avenue of the Hospital, Orléans-45100, France
Tel: +00330235952711
E-mail: [email protected]

Received Date: April 14, 2017; Accepted Date: April 25, 2017; Published Date: May 03, 2017

Citation: Larguech G, Cesaro A, Toumi H, Martinic I, Petoud S, et al. (2017) Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation. Anat Physiol 7:263. doi: 10.4172/2161-0940.1000263

Copyright: © 2017 Larguech G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Beta-(1 → 3)-glucans constitute highly promising biomolecules as evidenced by their immunostimulating ability which were first used as folk medicine in Chinese populations and further identified in fungi, yeasts and seaweeds. Previous investigations showed that glucans proteins interaction mediate cell growth pathways. We have adopted this approach to study the effect of laminarin, a beta-(1 → 3)-D-glucan, on Mesenchymal Stem Cells (MSCs) proliferation and differentiation. MSCs were cultured in MSC growth and chondrogenic differentiation mediums. Proliferation rate and apoptosis were explored by cell count, MTT assays and Annexin V staining. mRNA and protein expression of specifics markers for MSCs and chondrocytes were studied using qPCR and immunofluorescence. Results showed that laminarin treatment reduced MSCs proliferation in both growth and chondrogenic mediums (p<0.05). Annexin V staining showed no apoptosis. MSC cells in growth medium showed no impact of laminarin for Thy1, nucleostemin and endoglin mRNA analysis. Conversely, in chondrogenetic medium, laminarin had a negative effect on Thy1 levels and no change in nucleostemin and endoglin. Collagen II responded positively in chondrogenitic medium in absence of laminarin and significantly reduced when laminarin was added (p<0.05). These results indicate that laminarin inhibited both cells proliferation and chondrogenic differentiation suggesting potential clinical applications in MSC therapy.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords