Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte DifferentiationLarguech G1, Cesaro A1, Toumi H1,2*, Martinić I3, Petoud S3, Daniellou R4 and Lespessailles E1,2
- *Corresponding Author:
- Toumi H
Service of Rhumatology, Center Regional Hospital of Orléans
14 Avenue of the Hospital, Orléans-45100, France
E-mail: [email protected]
Received Date: April 14, 2017; Accepted Date: April 25, 2017; Published Date: May 03, 2017
Citation: Larguech G, Cesaro A, Toumi H, Martinic I, Petoud S, et al. (2017) Growth Inhibition of Mesenchymal Stem Cells by Laminarin: Impact on Chondrocyte Differentiation. Anat Physiol 7:263. doi: 10.4172/2161-0940.1000263
Copyright: © 2017 Larguech G, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Beta-(1 Ã¢ÂÂ 3)-glucans constitute highly promising biomolecules as evidenced by their immunostimulating ability which were first used as folk medicine in Chinese populations and further identified in fungi, yeasts and seaweeds. Previous investigations showed that glucans proteins interaction mediate cell growth pathways. We have adopted this approach to study the effect of laminarin, a beta-(1 Ã¢ÂÂ 3)-D-glucan, on Mesenchymal Stem Cells (MSCs) proliferation and differentiation. MSCs were cultured in MSC growth and chondrogenic differentiation mediums. Proliferation rate and apoptosis were explored by cell count, MTT assays and Annexin V staining. mRNA and protein expression of specifics markers for MSCs and chondrocytes were studied using qPCR and immunofluorescence. Results showed that laminarin treatment reduced MSCs proliferation in both growth and chondrogenic mediums (p<0.05). Annexin V staining showed no apoptosis. MSC cells in growth medium showed no impact of laminarin for Thy1, nucleostemin and endoglin mRNA analysis. Conversely, in chondrogenetic medium, laminarin had a negative effect on Thy1 levels and no change in nucleostemin and endoglin. Collagen II responded positively in chondrogenitic medium in absence of laminarin and significantly reduced when laminarin was added (p<0.05). These results indicate that laminarin inhibited both cells proliferation and chondrogenic differentiation suggesting potential clinical applications in MSC therapy.