Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in MiceOmar Suwaidan Ali1, Lawan Adamu1,4, Faez Firdaus Jesse Abdullah1,2*, Yusuf Abba3,4, Hazilawati Binti Hamzah3, Mohd-Azmi ML3, Abdul Wahid Haron1,2 and Mohd Zamri-Saad3
- *Corresponding Author:
- Faez Firdaus Jesse Abdullah
Department of Veterinary Clinical Studies
Universiti Putra Malaysia, 43400 UPM
Serdang, Selangor, Malaysia
Tel: +60 3-8609 3889
E-mail: [email protected]
Received date: November 03, 2014; Accepted date: February 24, 2015; Published date: February 27, 2015
Citation: Ali OS, Adamu L, Abdullah FFJ, Abba Y, Hamzah HB, et al. (2015) Haematological and Histopathological Vicissitudes Following Oral Inoculation of Graded Doses of Pasteurella multocida Type B: 2 and its Lipopolysaccharide in Mice. J Veterinar Sci Technol 6:220. doi:10.4172/2157-7579.1000220
Copyright: © 2015 Ali OS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Pasteurella multocida type B: 2 is responsible for major animal diseases of economic importance in both developed and developing countries. Haemorrhagic Septicaemia (HS) could inflict devastating effects on blood tissues and organs in the host animal. Therefore, the current study aims to investigate the haematological and clinico- pathological responses in mice following oral inoculation of graded doses of Pasteurella multocida type B: 2 and its lipopolysaccharide. Sixty healthy Balb c mice were placed in twelve plastic cages each one containing five mice. The mice were divided into three major groups (A, B and C). Group A is the control group (n=10) and these were inoculated with 0.4 ml of PBS pH 7.4 orally. The treatment groups (B; n=25 and C; n=25) were inoculated with P. multocida type B: 2 and its lipopolysaccharide respectively. The mice in group B and C were further divided into five subgroups. The subgroups were designated based on the graded doses as B101, B103, B105, B107 and B109 for Pasteurella multocida and C101, C103, C105, C107 and C109 for LPS respectively. The mice were observed for 120 hours post- inoculation. The clinical signs (Ruffled fur, Ocular discharges, Level of alertness and Laboured breathing) were significantly different (p<0.001) in mice inoculated orally with variable doses of Pasteurella multocida type B: 2 and its LPS. RBC, PCV, haemoglobin concentrations, PT, APTT, Thrombocyte, WBC, Lymphocytes, monocytes, plasma proteins, band and segmented neutrophils were significantly different (p<0.0001) in mice inoculated with graded doses of Pasteurella multocida type B: 2 and its LPS. Inflammatory cells, degeneration, necrosis and congestion were significantly different (p<0.0001) in mice inoculated with graded doses of both Pasteurella multocida type B: 2 and its LPS. In conclusion, 109 cfu of Pasteurella multocida type B: 2 and its lipopolysaccharide have devastating effects on organs and blood tissues.