Hemopressin an Inverse Agonist of Cb1 Cannabinoid Receptors Reverses Mechanical Sensitivity on Diabetes-Induced Neuropathy in MiceElaine FlamiaToniolo1,2, Adriano CardozoFranciosi1,2 and Camila Squarzoni Dale2*
- *Corresponding Author:
- Camila Squarzoni Dale
Department of Anatomy, University of São Paulo
Av. Prof. Lineu Prestes, 2415, S104B, 05508-900, São Paulo, Brazil
Tel/Fax: + 55 11 3091 0884
E-mail: [email protected]
Received date: February 25, 2014; Accepted date: March 24, 2014; Published date: March 27, 2014
Citation: Toniolo EF, Franciosi AC, Dale CS (2014) Hemopressin an Inverse Agonist of Cb1 Cannabinoid Receptors Reverses Mechanical Sensitivity on Diabetes-Induced Neuropathy in Mice. J Diabetes Metab 5:357. doi: 10.4172/2155-6156.1000357
Copyright: © 2014 Toniolo EF, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Peripheral neuropathy is one of the most common complications of diabetes affecting about 50% of patients with the disease. The most prominent symptoms involve the extremities and occur as both an exaggerated response to noxious stimuli (hyperalgesia), mild or non-painful stimuli (allodynia). Hemopressin (Hp) is a non apeptide first found in rat brain extracts, which selectively binds CB1 cannabinoid receptors (CB1R) and exerts antinociceptive actions in experimental inflammatory and neuropathic pain models. However there is no data about its efficacy in neuropathic metabolic-related disease, like diabetes mellitus. The aim of this study was to investigate the role of Hp on mechanical and thermal sensitivity of mice submitted to an experimental model of type 1 diabetes mellitus-induced neuropathy. Mechanical allodynia and thermal sensibility were assessed by von Frey filaments or plantar test, respectively, 7, 14, 21 and 28 days after streptozotocine injection (STZ; 200 mg/kg). Body weight and blood glucose were monitored once a week. Hp was administered orally, once a day (2.5 mg/kg) for 28 days. Hp reversed mechanical allodynia in diabetic mice without changing blood glucose levels or body weight. No effects were observed for thermal sensitivity. These results make hemopressin an attractive approach for the development of cannabinoid-based therapies for the treatment diabetic neuropathic pain.