Molecular Biology: Open Access

HER2I655V Polymorphims Detection by Non-Invasive Alelle-Specific PCR (AS-PCR)

Abstract

Arika Wahyuningsih, Bugi Ratno Budiarto, Eng Desriani and Tatag Bagus Putra Prakarsa

Allele-specific PCR for HER2I655V detection using breast cancer patients-obtained frozen tissue origin has been successfully developed. However, impassive approach to collect tissues hampers this method to be used for genetic screening purposes. Therefore, the present study is to test the use of buccal cells as an alternative for genetic material source in detecting HER2I655V polymorphism using AS-PCR. Firstly, we prepared standard marker of HER2I655V fragment with size of 142 bp for AA genotype and 168 bp for GG genotype using pGEM_HER2 recombinant as source of these fragment. These fragments then used as alternative of DNA ladder to estimate HER2I655V amplicon. AS-PCR using buccal cells has successfully performed to detect I655V polymorphism that showed AA genotype for all samples tested. In the next experiment we will conduct AS-PCR using this non-invasive method with more samples collected.