alexa Heterogeneity of The CD90+ Population in Different Stages of Hepatocarcinogenesis | OMICS International
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Journal of Proteomics & Bioinformatics
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Research Article

Heterogeneity of The CD90+ Population in Different Stages of Hepatocarcinogenesis

Smathorn Thakolwiboon1,3, Jianhui Zhu1, Qixing Liang2, Theodore H Welling1, Min Zhang2 and David M Lubman1*

1Department of Surgery, University of Michigan Medical Center, Ann Arbor, Michigan 48109, United States

2Department of Biostatistics, University of Michigan School of Public Health, Ann Arbor, Michigan 48109, United States

3Department of Medicine, Faculty of Medicine Siriraj hospital, Mahidol University, Bangkok10700, Thailand

*Corresponding Author:
David M Lubman
Department of Surgery, University of Michigan Medical Center
1150 West Medical Center Drive, Building MSRB1 Room A510B
Ann Arbor, MI, USA, 48109-0656
Tel: 734-647-8834
Fax: 724-615-2088
E-mail: [email protected]

Received Date: August 01, 2014; Accepted Date: September 08, 2014; Published Date: September 12, 2014

Citation: Thakolwiboon S, Zhu J, Liang Q, Welling TH, Zhang M, et al. (2014) Heterogeneity of The CD90+ Population in Different Stages of Hepatocarcinogenesis. J Proteomics Bioinform 7: 296-302. doi: 10.4172/jpb.1000332

Copyright: © 2014 Thakolwiboon S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Abstract

We have characterized herein the heterogeneity of the CD90+ population at each stage of hepatocarcinogenesis using a computer-assisted immunohistochemical staining evaluation method for quantitative analysis on tissue microarrays. We found that CD90 in Hepatocellular carcinoma (HCC) tissues, which has been shown to be a marker for cancer stem cells, is expressed on tumor cells, in the stroma or on endothelial cells. Sub-classification of the CD90+ population was based on morphology and co-expression with known markers including CD45 and CD31. Multiple linear regression suggested that the percentage of CD90+ cancer cells/hepatocyte (p<0.0001), level of overall CD90 expression (p<0.0014), and level of CD90 expression in tumor islands (p<0.0001) increased significantly in each stage of liver disease progression, while the level of stromal CD90 expression (p=0.1129) did not change significantly. Additionally, only the CD90+ cancer cells were positive for other cancer stem cell (CSC) markers including CD24, CD44 and CD133 whereas the other CD90+ cells were negative for these markers. CD90 expression in cirrhosis was observed in hepatocytes, the portal tract area and fibrous septa while CD90 expression in normal liver was limited only to the portal tract area. This study demonstrates the heterogeneity of the CD90+ population in HCC where a small population of the CD90+ cells that expressed other CSC markers are CSCs and are associated with advanced stages of hepatocarcinogenesis. This heterogeneity should be emphasized in further studies where other methods may not be able to discriminate these distinct types of CD90+ cells.

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