alexa HIF-1α and Resistance to Ionizing Radiation in Rectal Cancer
ISSN: 2161-1076

Surgery: Current Research
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

HIF-1α and Resistance to Ionizing Radiation in Rectal Cancer

Kelly Harris1,2, Xiaohuan Gao1,2, Sara Huerta-Yepez2, Payal Kapur2 and Sergio Huerta1,3*

1VA North Texas Health Care System, USA

2Department of Pathology, University of Texas Southwestern, USA

3Department of Surgery, University of Texas Southwestern, USA

*Corresponding Author:
Sergio Huerta, MD, FACS
VA North Texas Health Care System
Department of Surgery (112)
4500 S. Lancaster Road, Dallas, TX 75216, USA
Tel: 75390-9159
E-mail: [email protected]

Received date: June 05, 2014; Accepted date: July 16, 2014; Published date: July 18, 2014

Citation: Harris K, Gao X, Huerta-Yepez S, Kapur P, Huerta S (2014) HIF-1a and Resistance to Ionizing Radiation in Rectal Cancer. Surgery Curr Res 4:200. doi:10.4172/2161-1076.1000200

Copyright: © 2014 Harris K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Introduction: There is a wide range of responses to pre-operative chemoradiation (CRT) in patients with rectal cancer. Factors that dictate tumors response have not been identified. The objective of this study was to determine the role of hypoxia-inducible factor 1-alpha (HIF-1α) in in vitro and ex vivo models of rectal cancer. Methods: Levels of HIF-1α were assessed in radiosensitive (HCT-116) and a radioresistant (HT-29) cells. Radioresistant HT-29 cells were exposed to a potent radiosensitizing agent (a nitric oxide donor: DETANONOate [1 mM]) and IR followed by Western blot analysis with anti- HIF-1α antibodies. Patients with rectal cancer that were treated with CRT were separated based on good responders (≥ 50% reduction in tumor by comparing tumor size pre-operatively and postoperatively) compared to those that did not respond (<50% reduction in tumor). Blocks were stained for HIF-1α. Tissues collected from normal colonic epithelium from all these patients were subjected to the same treatments. Results: In vitro, radiosensitive HCT-116 colorectal cancer cells demonstrated low levels of HIF-1α following treatment IR. Western blot analysis showed high protein levels of HIF-1α in radioresistant HT-29 cells subjected to IR with induction at 2.0 and 4.0 Gy. Protein levels of HIF-1α were attenuated by treatment of cells HT-29 with the radiosensitizing agent DETANONOate following exposure to IR. Ex vivo, tissue microarrays demonstrated no difference in HIF-1α expression between patients with a poor response to CRT (418.1 ± 32.6) vs. good responders (371.1 ± 31.5; p=0.3). However, examination tumor tissue compared to normal epithelium showed an increase in HIF-1α (1.5 fold; p<0.001) in patients who responded poorly to IR. This effect was not observed in the cohort of patients who responded well to IR. Conclusions: The results of this study further support the potential role of HIF-1α in resistance to IR. This study implicates it a potential role of HIF-1α in rectal cancers.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]csonline.com

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords