alexa High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
Open Access

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Research Article

High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study

Saiqa Muneer1,2*, Iyad Naeem Muhammad1 and Muhammad Asad Abrar3

1School of Pharmacy, University of Queensland, Brisbane, Queensland, Australia

2The University of Lahore, Lahore, Pakistan

3The Islamia University of Bahawalpur, Bahawalpur, Pakistan

*Corresponding Author:
Saiqa Muneer
PhD Student, School of Pharmacy
University of Queensland, 20 Cornwall Street
Woollongabba, 4109, Brisbane
Queensland 4102, Australia
Tel: 0061469085227/469085227
Fax: 0469085227
E-mail: [email protected] (or) [email protected]

Received Date: May 27, 2017 Accepted Date: June 09, 2017 Published Date: June 14, 2017

Citation: Muneer S, Muhammad IN, Abrar MA (2017) High Performance Liquid Chromatographic Determination of Naproxen in Prepared Pharmaceutical Dosage Form and Human Plasma and its Application to Pharmacokinetic Study. J Chromatogr Sep Tech 8: 369. doi: 10.4172/2157-7064.1000369

Copyright: © 2017 Muneer S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A simple, fast, economical and precise reverse phase liquid chromatographic method was developed, optimized and validated for quantification of naproxen sodium according to the standard guidelines. The separation of the analyte and internal standard was achieved over C-18 column using acetonitrile: water: glacial acetic acid as mobile phase in a ratio of 50:49:1 (v/v) in isocratic mode at a flow rate of 1.2 mL/min at a wavelength of 254 nm at ambient temperature. The retention time was found to be less than 10 minutes. The limit of detection (LOD) was 10 ng/mL and limit of quantification (LOQ) was 15 ng/mL. Naproxen sodium was extracted from biological samples by using acetonitrile as extraction solvent. The linearity was found to be 0.5 to 80 ppm. All the parameters were validated for accuracy, precision, linearity, sensitivity, reproductivity and stability. The method was successfully applied for the quantification of the naproxen sodium in animal and human plasma. Therefore, the method was found to be accurate, reproducible, sensitive, cost effective, less time consuming and can be successfully applied on routine analysis of naproxen sodium in pharmaceutical formulations and pharmacokinetic studies in human and animal models.

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