High Throughput Optical Biosensor for Monitoring Pb (II) Ions in Milk through Fluorescence based Microarray Approach
- *Corresponding Author:
- Hardeep K
Biosensor Technology Laboratory
Department of Biotechnology
Punjabi University, Patiala, Punjab
E-mail: [email protected]
Received Date: March 14, 2015; Accepted Date: June 11, 2015; Published Date: June 21, 2015
Citation: Hardeep K, Verma N (2015) High Throughput Optical Biosensor for Monitoring Pb (II) Ions in Milk through Fluorescence based Microarray Approach. J Biosens Bioelectron 6:166. doi: 10.4172/2155-6210.1000166
Copyright: © 2015 Hardeep K, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Milk is a bulk consumer product therefore it serves a versatile source of public exposure to contaminants. Among the various heavy metals, lead has been recognized as the leading environmental health threat and milk has been found mostly contaminated with high levels of Pb (II) ions. In present study an optical biosensor is developed employing Bacillus sphaericus (MTCC 5100) as biorecognition unit and the analysis is based on urease inhibition resulting in fluorescence change. Investigation was carried out by means of fluorescence dye (Rhodamine 6G) in hydrophobic environment with the heavy metal. The novelty of the method lies in the formula that is devised to detect equivalent Pb (II) ions in milk in the presence of Cd (II). Lowest detection limit achieved is 0.48 nM Pb (II) equivalents in spiked milk samples (permissible limit 96.6 nM). This is the first report on low level Pb (II) monitoring in milk through high throughput microarray biosensing.