High-performance Liquid Chromatographic Method with Diode Array Detection for Quantification of Haloperidol Levels in Schizophrenic Patients During Routine Clinical Practice
- *Corresponding Author:
- Dr. Tarun Jain
Faculty of Pharmaceutical Sciences
Jodhpur National University, Jhawar Road
Boronada, Jodhpur-342001, Rajasthan, India
Tel: +91 9784155675
E-mail: [email protected]
Received Date: October 15, 2010; Accepted Date: November 11, 2010; Published Date: January 07, 2011
Citation: Jain T, Bhandari A, Ram V, Sharma S, Chaudhary RK, et al. (2011) High-performance Liquid Chromatographic Method with Diode Array Detection for Quantification of Haloperidol Levels in Schizophrenic Patients During Routine Clinical Practice. J Bioanal Biomed 3: 008-012. doi: 10.4172/1948-593X.1000037
Copyright: © 2011 Jain T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Rationale: Till date, very few HPLC methods are available with short run time for monitoring of haloperidol, facilitating large number of sample analysis within short time frame.
Objective: A selective and sensitive reverse phase high-performance liquid chromatographic assay has been developed for monitoring of Haloperidol levels.
Methodology: Chromatogram separation of Haloperidol and Loratidine (Internal Standard) was achieved using C18 column as stationary phase. Mobile phase consists of Acetonitrile and Water (50:50), pH-2.5 with 0.1% Acetic Acid and 0.05 M KHPO4 at a flow rate of 1.6 mL min-1. Detection was carried out at 240 nm using UV-PDA detector. Retention time for Haloperidol and Internal Standard was found to be 2:13 and 3:16 minutes respectively. The method has been validated for linearity, specificity, robustness, stability, accuracy and precision.
Results: Linearity for Haloperidol was in the range of 03-200 ng mL-1. The total run time of analysis was 5 minutes and the lower limits of detection and quantification were 1.0 and 3.0 ng mL-1 respectively. In present study, most of the enrolled patients were clinically stable on Haloperidol (as evident from various rating scales applied during the study period) at therapeutic range of 5-19 ng mL-1 [13.04 ± 4.03]
Discussion: This validated high-performance liquid chromatographic method using a simple mobile phase has been successfully applied for clinical monitoring of Haloperidol in psychiatric patients. The method is economic and its time sparing ability using simple RP-HPLC speaks its utility in clinical and toxicological management over other analytical methods.