Histological Analysis of Collagen Fibers in Patients with Diabetes Mellitus and Periodontal DiseasePopescu MR*, Surlin P, Rauten AM, Dragomir L and Olteanu M
Department of Prosthetics and Occlusology, Faculty of Dental Medicine, University of Medicine and Pharmacy of Craiova, Romania
- *Corresponding Author:
- Mihai Raul Popescu
Department of Prosthetics and Occlusology
Faculty of Dental Medicine, University of Medicine and Pharmacy of Craiova
2 Petru Rares Street, 200349 Craiova, Romania
Tel: +40722–557 407
E-mail: [email protected]
Received Date: April 03, 2014; Accepted Date: June 14, 2014; Published Date: June 16, 2014
Citation: Popescu MR*, Surlin P, Rauten AM, Dragomir L, Olteanu M (2014) Histological Analysis of Collagen Fibers in Patients with Diabetes Mellitus and Periodontal Disease. J Cytol Histol S4:008. doi:10.4172/2157-7099.S4-008
Copyright: © 2014 Popescu MR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The researchers suggest that diabetes increases the degradation of the newly synthesized collagen from the conjunctive tissue. The altered metabolism of the collagen may predispose the diabetic patients not only to periodontal disease, but also to some other anomalies such as defective healing. The purpose of the study was to go through a histological analysis of the collagen fibers from the chorion in patients with diabetes mellitus and periodontal disease.
Materials and Methods: In our study we examined a total of 68 patients diagnosed with diabetes mellitus type 1 and 2. In these patients we achieved simple gingivectomy or tooth extraction in order to obtain gingival tissue fragments. The preparations included at paraffin and then stained with Hematoxylin-Eosin and trichromic after Goldner-Szeckeli method.
Results: In the patients with diabetes mellitus with an evolution of less than 10 years the collagen fibers appeared as dissociated by the inflammatory cells that were inserted among them. It was also noted the fragmentation of collagen fibers and sometimes even their lysis with subepithelial connective tissue disorganization. In patients with diabetes for over 10 years there has been noticed a plentiful collagen sclerosis, zones of collagen hyalinization with an increased number of fibrocytes.
Conclusion: The extracellular matrix is altered in the investigated patients with destructive lesions of collagen or collagen sclerosis.