Histological and Immunohistological Evaluation of the Tissue Response of a New Barrier Material Based on D,L-Polylactide, Trimethylene Carbonate and Caprolactone to Prevent Peritoneal Adhesion Formation
Constanze NE Planck1,5, Volker H Schmitt2, Bernhard Krämer1, Andreas Mamilos2, Taufiek K Rajab3, David Hollemann2, Christian Wallwiener1, Helmut Hierlemann4,5, Heinrich Planck4,5, C James Kirkpatrick2 and Christoph Brochhausen2*
- Corresponding Author:
- Christoph Brochhausen
REPAIR-lab, Institute of Pathology, University Medical Center
Johannes Gutenberg University, Mainz, Germany
Tel: ++49 06131 17 73 07
Fax: ++49 06131 17 47 73 07
E-mail: [email protected]
Received date: March 07, 2014; Accepted date: May 20, 2014; Published date: May 23, 2014
Citation: Planck CNE, Schmitt VH, Krämer B, Mamilos A, Rajab TK, et al. (2014) Histological and Immunohistological Evaluation of the Tissue Response of a New Barrier Material Based on D,L-Polylactide, Trimethylene Carbonate and Caprolactone to Prevent Peritoneal Adhesion Formation. J Tissue Sci Eng 5:138. doi:10.4172/2157-7552.1000138
Copyright: © 2014 Planck CNE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Serosal membranes cover body cavities and guarantee frictionless gliding of inner organs. Serosal damage carries the risk of adhesion formation, which represents a relevant postoperative complication. Due to pathophysiological similarities between serous and dermal wound healing, the efficacy of SupraSeal® in intraabdominal adhesion prevention was investigated at early time points. After standardized serosal damage, Wistar rats were examined macroscopically, histologically and immunohistochemically on days two, four and eight postoperatively. On day eight, all specimens of the control group presented distinctive adhesions consisting of fibrous tissue bands. In contrast, most specimens covered by SupraSeal® were adhesion-free. Merely mild adhesion formation due to suture material used for fixation was detected. Histologically, SupraSeal® revealed rapid clearance of fibrin and a marked lack of fibrosis. For the first time, the histomorphological effects of SupraSeal® on adhesion formation over time are described and the relevance of the early stage of wound healing elucidated.