Histological and Transcriptional Expression differences between Diabetic Foot and Pressure Ulcers
Yssel Mendoza-Mari1, Calixto Valdés-Pérez2, Elena Rodríguez-Corrales3, José Suárez-Alba4, Ariana García-Ojalvo1, Diana García del Barco1, Gerardo Guillén-Nieto1, Luis Herrera-Martínez1 and Jorge Berlanga-Acosta1*
- *Corresponding Author:
- Jorge Berlanga-Acosta
Wound Healing and Cyto-Protection Research Group
Biomedical Research Direction
Center for Genetic Engineering and Biotechnology
Havana 10600, PO Box: 6162, Havana, Cuba
Tel: 53-7-271 60 22
Fax: 53-7-250 44 94
E-mail: [email protected]
Received date: September 02, 2013; Accepted date: October 07, 2013; Published date: October 12, 2013
Citation: Mendoza-Mari Y, Valdés-Pérez C, Rodríguez-Corrales E, Suárez-Alba J, García-Ojalvo A, et al. (2013) Histological and Transcriptional Expression differences between Diabetic Foot and Pressure Ulcers. J Diabetes Metab 4:296. doi:10.4172/2155-6156.1000296
Copyright: © 2013 Mendoza-Mari Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Decubitus and diabetic foot ulcers remain as important clinical challenges with significant socioeconomic impact. Both are individual forms of chronic wounds with diverse proximal ethiopathogenic triggers. This study aimed to characterize and compare the main histological features as the transcriptional expression profile of a set of wound-healing relevant genes of the ulcers’ granulation tissue. Following patients’ consent, biopsies were collected from sacrolumbar pressure ulcers (N=5, stage IV) and diabetic foot ulcers (N=9, both of neuropathic and ischemic origin) with clean, non-infected granulation tissue. Biopsies fragments were processed for histological analysis and for RNA extraction and subsequent transcriptional expression characterization via RT-PCR. The group of targeted genes included cell proliferation control, extracellular matrix, glucose metabolism, anabolismsurvival, as anti-hypoxia and anti-oxidant defense. Gene expression was determined, normalized with an internal housekeeping gene, and statistically compared. Each class of chronic ulcer granulation tissue: decubitus, and diabetics’ ischemic and neuropathic proved to develop a particular histological pattern thus establishing individual differences. Moreover, diabetes appeared to significantly reduce the expression of numerous genes irrespective to their biological significance. Most importantly, we found that diabetic granulation tissue cells exhibit a sort of “genetic or epigenetic imprinting” for the expression of glucose-metabolism related genes which are deeply involved in type-2 diabetes pathophysiology. Our data indicate that in addition to a protracted inflammation and abnormal angiogenesis, diabetic granulation tissue cells are affected by gene expression failures that may lead to a negative pro-anabolic and energetic balance.