Histone Deacetylase Inhibitors can Repress Proliferation and Induce Apoptosis in Gingiva Progenitor Cells from Idiopathic Gingival FibromatosisZhi Jia1#, Chunyue Feng1,2#, Yingchenyao Wang1, Jingkun Li1, Tingting Zhang3, Jian Zhang3 and Dayong Liu1*
- *Corresponding Author:
- Dayong Liu, DDS, PhD
Department of Endodontics & Laboratory for Dental Stem Cells and Endocrine Immunology
Tianjin Medical University School of Stomatology, Qi Xiang Tai Road No.12
Tianjin, 300070, PR China
E-mail: [email protected]
Received date: January 13, 2016 Accepted date: February 05, 2016 Published date: February 12, 2016
Citation: Jia Z, Feng C, Wang Y, Li J, Zhang T, et al. (2016) Histone Deacetylase Inhibitors can Repress Proliferation and Induce Apoptosis in Gingiva Progenitor Cells from Idiopathic Gingival Fibromatosis. J Stem Cell Res Ther 6:327. doi:10.4172/2157-7633.1000327
Copyright: © 2016 Jia Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: Idiopathic gingival fibromatosis (IGF), which is characterized by diffuse enlargement of the gingiva due to expansion and accumulation of connective tissue, is a relatively rare hereditary condition that has no specific cause. The aim of this study was to investigate whether the anti-tumor drugs trichostatin A (TSA; inhibits histone deacetylase) and panobinostat (LBH589; a non-selective inhibitor of histone deacetylase) inhibit the proliferation of cells derived from gingiva in patients with IGF.
Materials and methods: After osteogenic differentiation and adipogenic induction, the differences between normal gingival mesenchymal stem cells ( N-GMSCs ) and hyperplastic gingival mesenchymal stem ( H-GMSCs, theI cells from IGF ) were determined in terms of alkaline phosphatase staining and activity, alizarin red staining, calcium-nodule formation and, lipid droplets,. The effects of TSA and LBH589 were investigated via the MTT assay, flow cytometry, and reverse transcription polymerase chain reaction (RT-PCR).
Results: Alkaline phosphatase staining and activity, alizarin red staining, lipid droplets detected the differentiative capacity in normal and IGF cells. These assays indicated that IGF cells possess multipotent differentiation properties similar to those of normal cells. RT-PCR showed that mRNA levels of the gene encoding p21Waf/Cip1, a cyclin-dependent kinase inhibitor and an essential regulator of growth inhibition, were lower in H-GMSCs than in N-GMSCs. After exposure to 1000 nM TSA or 1000 nM LBH589 for 48 h, p21Waf/Cip1 mRNA levels increased in H-GMSCs.
Conclusions: TSA and LBH589 can repress the growth and proliferation of hyperplastic IGF cells by regulating p21Waf/Cip1 mRNA levels.