Homology Modeling and Functional Analysis of LPG2 Protein of Leishmania Strains
- *Corresponding Author:
- Dr. Ganesh Chandra Sahoo
Rajendra Memorial Research Institute of Medical Sciences
Patna, Bihar, INDIA
E-mail: [email protected]
Received Date: November 27, 2008; Accepted Date: January 06, 2009; Published Date: January 10, 2009
Citation: Ganesh CS, Manas RD, Mukta R, Pradeep D (2009) Homology Modeling and Functional Analysis of LPG2 Protein of Leishmania Strains. J Proteomics Bioinform 2:032-050. doi:10.4172/jpb.1000059
Copyright: © 2009 Ganesh CS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
As drug resistance problem persists in case of Leishmaniasis, modeling and analysis of different essential proteins of Leishmania strains will help us further to discover novel lead compounds. Lipophosphoglycan 2 (LPG2) protein is required for the development of Leishmania throughout their life cycle, including for virulence to the mammalian host. LPG2 participates in a specialized virulence pathway, which may offer an attractive target for chemotherapy. Homology models of LPG2 of five Leishmania species have been constructed using the X-ray structures of different transporter proteins as templates, by comparative protein modeling principles. The resulting model has the correct stereochemistry as gauged from the Ramachandran plot and good three-dimensional (3-D) structure compatibility as assessed by the Procheck and Profiles-3D scores. Functional assignment of LPG2 protein of Leishmania strains by SVM revealed that along with transporters activity it also performs several novel functions e.g. iron-binding, sodium-binding, copper binding. It also belongs to protein of major facilitator family (MFS) and type II (general) secretory pathway (IISP) family. Important functional motifs have been identified in LPG2 protein of different Leishmania strains using different programs. Potential Ligand Binding Sites (LBSs) in LPG2 protein of these strains have been identified using Pocket Finder program. On the basis of structure of ligand binding sites, particular LPG2 inhibitors can be designed. The similarity in the molecular structure, function and differences in LBSs of LPG2 of L. donovani, L. major, L. infantum, L. braziliensis and L. mexicana provide evidences for selective and specific LPG2 inhibitors.