Homology Modelling of Hypoxanthin–Guanine Phosphoribosyltransferase, Enzyme Involved in Salvage Pathway of Purine MetabolismJyotsna Choubey1*, Ashish Patel1, Sheetal Khatri1, Ruby Dewangan1, Shailendra Kumar Gupta2, M.K. Verma1
- *Corresponding Author:
- Dr. Jyotsna choubey,
Sub -DIC Bioinformatics center,
NIT Raipur (C.G.) 492010, India,
E-mail: [email protected], [email protected]
Received date: August 06, 2009; Accepted date: September 22, 2009; Published date: September 25, 2009
Citation: Choubey J, Patel A, Khatri S, Dewangan R, Gupta SK, et al. (2009) Homology Modell ing of Hypoxanthin-Guanine Phosphoribosyltransferase, Enzyme Involved in Salvage Pathway of Purine Metabolism. J Comput Sci Syst Biol 2:259-261. doi:10.4172/jcsb.1000039
Copyright: © 2009 Choubey J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Hypoxanthin-guanine phosphoribosyltransferase (HPRT) is responsible for catalyzing a reaction which breaks down uric acid in the body. The lack of this enzyme creates a build-up of uric-acid, leading to a neurological problem, joint pain and kidney problem. The condition is called Lesch-Nyhan Syndrome. A comparative modelling of the protein Hypoxanthin-guanine phosphoribosyltransferase is performed using the modeling program modeller9v3.The following structure validation programs PROCHECK, PROSA, VERIFY3D and WHATIF are used. Descret Optimized Protein Energy (DOPE), is a statistical potential used to asses homology model in protein structure prediction. Predicted model can be useful to develop new inhibitor against Lesch-Nyhan Syndrome. CASTp can be used to study surface features and functional regions of proteins.