alexa How Should the World Manage the Challenge of Diabetes Mellitus
ISSN: 2327-5146

General Medicine: Open Access
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Commentary

How Should the World Manage the Challenge of Diabetes Mellitus

Abdullah Nasrat M*
Department of Surgery, Balghsoon Clinic, Jeddah, KSA
Corresponding Author : Abdullah Nasrat M
Department of Surgery, Balghsoon Clinic, P.O. Box 52611
Jeddah 21573, KSA
Tel: +966 (012) 667 3645
Fax: +966 (012) 667 3645
E-mail: [email protected]
Received: December 30, 2015; Accepted: January 30, 2016; Published: February 02, 2016
Citation: Nasrat MA (2016) How Should the World Manage the Challenge of Diabetes Mellitus. Gen Med (Los Angel) 4:223. doi:10.4172/2327-5146.1000223
Copyright: © 2016 Nasrat MA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important pathogens that cause nosocomial infections. However, microbiological culture techniques take a few days to yield results; therefore, a simple, cost-effective, and rapid detection system is required for screening for MRSA and related bacteria: Methicillinresistant Staphylococcus epidermidis (MRSE) carriers during the hospital admissions process. In this study, we described the simplified method using by one-time use and screen-printed carbon electrodes, relied upon current quantification of Hoechst dyes which bound with DNA amplified via polymerase chain reaction (PCR) targeted for MRSA mecA gene. Amount of DNA-bound Hoechst molecules were measured by the hand-held potentiostat within two minutes. We found that the peak of a Hoechst-mediated current depended upon the number of MRSA cells, and successfully distinguished between carriers and a non-carrier based on nasal swabs from the patients. This method required only 10 μL for application, and the results could be obtained within total 60 min from sample collection when a minimum of 1 × 103 MRSA cells was present. These results suggested that this minimized technique has the potential to become a useful system of active surveillance for MRSA/MRSE carriers.

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