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HPLC Analysis of Monomer Release from Conventionally and High Temperature High-Pressure Polymerised Urethane Dimethacrylate Intended for Biomedical Applications | Abstract
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

HPLC Analysis of Monomer Release from Conventionally and High Temperature High-Pressure Polymerised Urethane Dimethacrylate Intended for Biomedical Applications

Mie-leng Tang1, Jean-François Nguyen1, Michaël Sadoun1 and N Dorin Ruse2*

1Unité de Recherches Biomatériaux Innovants et Interfaces (URB21-EA4462), Faculté de chirurgie dentaire, Université Paris Descartes, Sorbonne Paris Cité, Paris, France

2Faculty of Dentistry, University of British Columbia, Vancouver, Canada

*Corresponding Author:
N Dorin Ruse
Faculty of Dentistry
University of British Columbia
Vancouver, Canada
Tel: 1-604-822-4438
Fax: 1-604-822-3562
E-mail: [email protected]

Received date: May 15, 2014; Accepted date: June 27, 2014; Published date: June 30, 2014

Citation: Tang M, Nguyen J, Sadoun M, Dorin Ruse N (2014) HPLC Analysis of Monomer Release from Conventionally and High Temperature High-Pressure Polymerised Urethane Dimethacrylate Intended for Biomedical Applications. J Chromatograph Separat Techniq 5:227. doi:10.4172/2157-7064.1000227

Copyright: © 2014 Tang M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Since monomer release poses significant biocompatibility concerns, the aim of this study was to determine, using HPLC, and compare monomer release from conventionally and high-temperature high-pressure (HT/HP) polymerized urethane dimethacrylate (UDMA) for biomedical applications. Three polymers were made: a) a control, obtained by conventional thermo-polymerization of UDMA with 0.5% (w) benzoyl peroxide (BPO) as initiator; b) an experimental, obtained by HT/HP polymerization of UDMA with 0.5% (w) BPO; and c) another experimental, obtained by HT/HP polymerization of UDMA without initiator. Bar-shaped polymer specimens were immersed in HPLC-grade 75% ethanol for 1 d, 7 d, 14 d, and 28 d prior to monomer determination by HPLC with an Agilent 1260 Infinity Quaternary LC. A Poroshell 120 EC-C18 (4.6x50 mm; 2.7μm) column and elution solvent consisting of HPLC-grade 65% acetonitrile in water, with a flow rate of 1 μL/min, were used. A calibration curve was constructed using standard UDMA solutions in the range of 1x10-5 M to 1x10-7 M. The limits of detection (LOD=2.62x10-6 M) and quantification (LOQ=7.65x10-6 M) for UDMA were determined. The accuracy of the method was confirmed by standard additions. Monomer release was statistically higher in the control group at all-time intervals; the lowest release was detected in the BPO-containing HT/HP polymerized group. The results suggested that there was a significant reduction in free monomer content in HT/HP polymerized UDMA and that polymers obtained under HT/ HP conditions could be more biocompatible


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