HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma
Nehad A Abdallah*
Experiments and Advanced Pharmaceutical Research Unit (EAPRU), Faculty of Pharmacy, Ain Shams University, Egypt
- *Corresponding Author:
- Abdallah NA
Experiments and Advanced Pharmaceutical Research Unit (EAPRU)
Faculty of Pharmacy, Ain Shams University, Egypt
Tel: 020 01146447587- 020 01001353678
E-mail: [email protected]
Received date: April 07, 2014; Accepted date: April 26, 2014; Published date: April 28, 2014
Citation: Abdallah NA (2014) HPLC and Densitometric TLC Methods for Simultaneous Determination of Gemifloxacin with Some Co-administered Drugs in Human Plasma. J Chromatograph Separat Techniq 5:220. doi:10.4172/2157-7064.1000220
Copyright: © 2014 Abdallah NA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Two chromatographic methods have been developed for determination of gemifloxacin in human plasma with three co-administered drugs, theophylline, warfarin and omeprazole. First method depends on reverse phase high performance liquid chromatography. The plasma sample was extracted using acetonitrile. The method was linear over the concentration range 0.05 to 6 μg/mL, 0.25 to 8 μg/mL, 0.1 to 10 μg/mL and 0.1 to 6 μg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase used was prepared by mixing acetonitrile and 0.02 mol L-1 potassium dihydrogen phosphate buffer (pH adjusted to 2.5 using ortho phosphoric acid) in a ratio 10:90 with the addition of 1% TEA and flow rate 1 mL/min in isocratic mode and UV-detection at wavelength 254 nm. Second method depends on densitometric thin layer chromatography. The method was linear over concentration range 0.1 to 3 μg/ml, 0.5 to 6 μg/mL, 0.2 to 2.5 μg/mL and 0.1 to 1.5 μg/mL of gemifloxacin mesylate, theophylline, warfarin and omeprazole, respectively. The mobile phase was selected as mixture of dichloromethane, methanol and ammonia in the ratio of (7: 5.5: 3 v/v/v) for the development of plates. Densitometric analysis was carried out at wavelength 254 nm. The stability of gemifloxacin mesylate and the co-administered drugs in plasma was confirmed during three freeze–thaw cycles (−20°C).