HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples
- *Corresponding Author:
- Malik Alamgir
Institute of Advanced Research Studies in Chemical Sciences
University of Sindh, Jamshoro, Pakistan
Tel: +92 3465092589
Email: [email protected]
Received Date: March 29, 2017; Accepted Date: May 11, 2017; Published Date: May 18, 2017
Citation: Alamgir M, Khuhawar MY, Memon SQ, Hayat A, Zounr RA, et al. (2017) HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples. Pharm Anal Acta 8:546. doi: 10.4172/2153-2435.1000546
Copyright: © 2017 Alamgir M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A novel High Performance Liquid Chromatography (HPLC) method has been developed based on pre column derivatization with benzoin for determination of metformin, famotidine and ranitidine. The separation was achieved from C18 column when eluted isocratically, the solution of the drugs with methanol, water, acetonitrile and Tetra Hydrofuran (THF) (40:40:16:4 v/v) with a flow rate at 1 mL/min. UV detection was found to be 268 nm. Linear calibrations range was obtained with 2.5-12.5 μg/ml, with limits of detection (LODs) 0.091-0.30 μg/ml. The total run time for elution was 3.5 min. The derivitization, separation and quantitation was repeatable (n=4) in terms of retention time and peak height/peak area with Relative Standard Deviation (RSD) within 0.84-1.55% and 0.68-1.17% respectively. The method was applied for the analysis of metformin, famotidine and ranitidine from pharmaceutical preparations, human serum and human urine. The possible interfering effects of the sample matrix were checked by the analysis by standard addition method and matrix effect was not indicated.