alexa HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples
ISSN : 2153-2435

Pharmaceutica Analytica Acta
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples

Malik Alamgir1*, Muhammad Yar Khuhawar1, Saima Qayoom Memon2, Amir Hayat1, Rizwan Ali Zounr1 and Asma Chanar1

1Institute of Advanced Research Studies in Chemical Sciences, University of Sindh, Jamshoro, Pakistan

2Institute of Chemistry, University of Sindh, Jamshoro, Pakistan

*Corresponding Author:
Malik Alamgir
Institute of Advanced Research Studies in Chemical Sciences
University of Sindh, Jamshoro, Pakistan
Tel: +92 3465092589
Email: [email protected]

Received Date: March 29, 2017; Accepted Date: May 11, 2017; Published Date: May 18, 2017

Citation: Alamgir M, Khuhawar MY, Memon SQ, Hayat A, Zounr RA, et al. (2017) HPLC Determination of Metformin, Famotidine and Ranitidine by Derivatization with Benzoin from Drugs and Biological Samples. Pharm Anal Acta 8:546. doi: 10.4172/2153-2435.1000546

Copyright: © 2017 Alamgir M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A novel High Performance Liquid Chromatography (HPLC) method has been developed based on pre column derivatization with benzoin for determination of metformin, famotidine and ranitidine. The separation was achieved from C18 column when eluted isocratically, the solution of the drugs with methanol, water, acetonitrile and Tetra Hydrofuran (THF) (40:40:16:4 v/v) with a flow rate at 1 mL/min. UV detection was found to be 268 nm. Linear calibrations range was obtained with 2.5-12.5 μg/ml, with limits of detection (LODs) 0.091-0.30 μg/ml. The total run time for elution was 3.5 min. The derivitization, separation and quantitation was repeatable (n=4) in terms of retention time and peak height/peak area with Relative Standard Deviation (RSD) within 0.84-1.55% and 0.68-1.17% respectively. The method was applied for the analysis of metformin, famotidine and ranitidine from pharmaceutical preparations, human serum and human urine. The possible interfering effects of the sample matrix were checked by the analysis by standard addition method and matrix effect was not indicated.


Share This Page

Additional Info

Loading Please wait..
Peer Reviewed Journals
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals


[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version