HPLC-MS/MS Based Time Course Analysis of Morphine and Morphine-6- Glucoronide in ICU Patients
Eisenried A*, Peter J, Lerch M, Schüttler J and Jeleazcov C
Department of Anesthesiology, University of Erlangen-Nurnberg, Krankenhausstrabe 12, 91054 Erlangen, Germany
- *Corresponding Author:
- Andreas E
Department of Anesthesiology
University of Erlangen-Nurnberg
Erlangen, Krankenhausstrabe 12
91054 Erlangen, Germany
E-mail: [email protected]
Received date: May 15, 2017; Accepted date: May 31, 2017; Published date: June 01, 2017
Citation: Eisenried A, Peter J, Lerch M, Schüttler J, Jeleazcov C (2017) HPLC-MS/MS Based Time Course Analysis of Morphine and Morphine-6-Glucoronide in ICU Patients. J Chromatogr Sep Tech 8:368. doi: 10.4172/2157-7064.1000368
Copyright: © 2017 Eisenried A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Morphine is a widely-used opioid analgesic to treat post-operative pain in the intensive care unit. For the quantification of morphine and its metabolite Glucuronide (M6G) concentrations a sensitive and specific liquid chromatography–tandem mass spectrometry (LC-MS/MS) method was developed and validated according to Food and Drug Administration (FDA) guidelines. Plasma samples were extracted with solid-phase extraction and substituted with deuterated morphine and M6G as internal standards. Separation was performed by gradient elution using UPLC-like system and analyzed by MS/MS consisting of an electrospray ionization source. The lower limit of quantification was 500 ‘‘pg/ml’’ for morphine and 50 ‘‘pg/ml’’ for M6G. Intra- and interassay precision and accuracy did not exceed ± 15%. The method was applied to a clinical study during intensive care treatment of patients after coronary artery bypass grafting and can serve for further pharmacokinetic studies.