alexa HPLC-UV Analysis of Phloretin in Biological Fluids and
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies

Connie M. Remsberg1, Jaime A. Yáñez1, Karina R. Vega-Villa1, Nicole D. Miranda2, Preston K. Andrews3 and Neal M. Davies1*

1College of Pharmacy, Department of Pharmaceutical Sciences, Washington State University, Pullman, Washington, USA

2Facultad de Ingeniería Química, Universidad de Concepción, Concepción, Chile

3Department of Horticulture, Washington State University, Pullman, Washington, USA

*Corresponding Author:
Neal M Davies
College of Pharmacy
Department of Pharmaceutical Sciences
Washington State University, Pullman
Washington, USA
Tel: 509 335-4754
Fax: 509 335-5902
E-mail: [email protected]

Received date: August 11, 2010; Accepted date: September 09, 2010; Published date: September 11, 2010

Citation: Remsberg CM, Yáñez JA, Vega-Villa KR, Davies NM, Andrews PK, et al. (2010) HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies. J Bioremed Biodegrad 1:101. doi:10.4172/2157-7064.1000101

Copyright: © 2010 Remsberg CM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A method of analysis of phloretin [3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one] in biological fl uids is necessary to study the kinetics of in vitro and in vivo metabolism and its concentration in natural products. A high- performance liquid chromatographic (HPLC) method was developed for the determination of phloretin in rat serum. Separation was achieved on a Chiralcel ® OD-RH column with UV detection at 288 nm. The calibration curves were linear ranging from 0.5 to 100  g/ml. The mean extraction ef fi ciency was >95%. Precision of the assay was <14%, and was within 10.9% at the limit of quantitation (0.5  g/ml). Bias of the assay was lower than 14%, and was within 9.22% at the limit of quantitation. The HPLC method was successfully applied to the pharmacokinetic study of phloretin in rats.


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