HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic StudiesConnie M. Remsberg1, Jaime A. Yáñez1, Karina R. Vega-Villa1, Nicole D. Miranda2, Preston K. Andrews3 and Neal M. Davies1*
- *Corresponding Author:
- Neal M Davies
College of Pharmacy
Department of Pharmaceutical Sciences
Washington State University, Pullman
Tel: 509 335-4754
Fax: 509 335-5902
E-mail: [email protected]
Received date: August 11, 2010; Accepted date: September 09, 2010; Published date: September 11, 2010
Citation: Remsberg CM, Yáñez JA, Vega-Villa KR, Davies NM, Andrews PK, et al. (2010) HPLC-UV Analysis of Phloretin in Biological Fluids and Application to Pre-Clinical Pharmacokinetic Studies. J Bioremed Biodegrad 1:101. doi:10.4172/2157-7064.1000101
Copyright: © 2010 Remsberg CM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A method of analysis of phloretin [3-(4-hydroxyphenyl)-1-(2,4,6-trihydroxyphenyl)propan-1-one] in biological fl uids is necessary to study the kinetics of in vitro and in vivo metabolism and its concentration in natural products. A high- performance liquid chromatographic (HPLC) method was developed for the determination of phloretin in rat serum. Separation was achieved on a Chiralcel ® OD-RH column with UV detection at 288 nm. The calibration curves were linear ranging from 0.5 to 100 Ã¯ÂÂ g/ml. The mean extraction ef fi ciency was >95%. Precision of the assay was <14%, and was within 10.9% at the limit of quantitation (0.5 Ã¯ÂÂ g/ml). Bias of the assay was lower than 14%, and was within 9.22% at the limit of quantitation. The HPLC method was successfully applied to the pharmacokinetic study of phloretin in rats.