Human C-peptide Quantitation by LC-MS Isotope-Dilution Assay in Serum or Urine Samples
- *Corresponding Author:
- Alexander V Stoyanov
Department of Pathology and Anatomical Sciences
School of Medicine, University of Missouri
1 Hospital Dr., MSB Room M711, Columbia, MO 65212, USA
E-mail: [email protected]
Received Date: March 12, 2013; Accepted Date: March 23, 2013; Published Date: March 27, 2013
Citation: Stoyanov AV, Connolly S, Rohlfing CL, Rogatsky E, Stein D, et al. (2013) Human C-peptide Quantitation by LC-MS Isotope-Dilution Assay in Serum or Urine Samples. J Chromat Separation Techniq 4:172. doi: 10.4172/2157-7064.1000172
Copyright: © 2013 Stoyanov AV, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
In this communication we report a simple and efficient approach to C-peptide quantitation using isotope dilution mass-spectrometry analysis. The method facilitates quantitation of C-peptide levels at least one order of magnitude lower compared to concentration levels achieved with IDA method reported previously. The improvement was due to more intensive sample preparation procedure that, in turn, makes it possible to increase the sample load without a corresponding increase in matrix effects. We also show the results of a comparison study with a second laboratory using a similar previously reported method for C-peptide quantitation.