Human Embryonic Behavior Observed with Time-Lapse CinematographyYasuyuki Mio1*, Kyoko Iwata1, Keitaro Yumoto1 and Kazuo Maeda2
- *Corresponding Author:
- Yasuyuki Mio
Reproductive Centre, Mio Fertility Clinic
2-1-1 Kuzumo-Minami, Yonago, Japan
E-mail: [email protected]
Received Date: December 16, 2013; Accepted Date: January 04, 2014; Published Date: January 10, 2014
Citation: Mio Y, Iwata K, Yumoto K, Maeda K (2014) Human Embryonic Behavior Observed with Time-Lapse Cinematography. J Health Med Informat 5:143. doi: 10.4172/2157-7420.1000143
Copyright: © 2014 Mio Y, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
To clarify developmental behavior in human embryos, we observed oocytes with in vitro fertilization and intracytoplasmic sperm injection and donated embryos up to 5 days using our original time-lapse cinematography (TLC) system. The TLC analyses demonstrated several novel aspects and events that were not visible by intermittent stillframe microscopy. Here, we present the entire fertilization process in which we founded the fertilization cone, cytoplasmic flare, dynamic change of nuclear precursor body and cytoplasmic halo. We also demonstrated the occurrence of zygotes with a single pronucleus (PN) and uneven 2PN, fragmentation, blastocoel collapse during the blastocyst stage, and the hatching pattern of expanded blastocysts. Observing such morphokineses in human embryos will enhance our understanding of human embryonic behavior up to preimplantation.