alexa Human Papillomavirus 16 L1 Protein Expression and Self-
ISSN: 2229-8711

Global Journal of Technology and Optimization
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Research Article

Human Papillomavirus 16 L1 Protein Expression and Self-Assembly in Recombinant Pichia pastoris

Jing Zhao1, Ze-jian Wang1*, Hai-feng Hang1, Mei-jin Guo1*, Ying-ping Zhuang1, Ju Chu1, Si-liang Zhang1 and Jue-ren Lou2

1State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, P.R. China

2No. 2 Research Laboratory, Shanghai Institute of Biological Products Co., Ltd., Shanghai 200052, China

Corresponding Author:
Ze-Jian Wan
State Key Laboratory of Bioreactor Engineering
East China University of Science & Technology 329
130 Meilong Road, Shanghai 20023, People’s Republic of China
Fax: +86-21-64252252
E-mail: [email protected]
Meijin Guo
State Key Laboratory of Bioreactor Engineering
East China University of Science and Technology 329
130 Meilong Road, Shanghai 200237, People’s Republic of China
Fax: +86 21 64253702
E-mail: [email protected]

Received date: November 30, 2014; Accepted date: December 02, 2014; Published date: December 12, 2014

Citation: Zhao J, Wang ZJ, Hang HF, Guo MJ, Zhuang YP, et al. (2015) Human Papillomavirus 16 L1 Protein Expression and Self-Assembly in Recombinant Pichia pastoris. Global J Technol Optim 6:168. doi: 10.4172/2229-8711.1000168

Copyright: © 2015 Zhao J, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Human papillomavirus virus-like particles (HPV VLPs) have been shown to be effective in preventing cervical cancer when used as a prophylactic vaccine. However, little attention has been paid to the process of L1 expression and self-assembly of VLPs in vivo. In this study, the methanol concentration is optimized during fermentation to optimize L1 expression and self-assembly into VLPs. HPV 16 L1 was expressed in Pichia pastoris GS115, and successfully self-assembled into HPV 16 VLPs. The results demonstrate that there is a strong correlation between methanol concentration and the expression of HPV L1 as well as VLP formation. During the methanol induction phase, low concentrations of residual methanol (0.1-0.3%) promoted the expression of free L1 and increased the total content of L1, whereas a higher residual methanol concentration (0.32-0.6%) was conducive for the self-assembly of LI into VLPs. To conclude, if the residual methanol concentration is too low or too high, the levels of L1 can easily decrease during the fermentation process.


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