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ISSN: 2167-0501

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Research Article

Hydroxyl Radical Generation by Mammalian Catalase: A Few Experimental Evidences

Madhur M Goyal*, Ajay Meshram and Anjan Basak
Department of Biochemistry, J.N. Medical College, Datta Meghe Institute of Medical Sciences (Deemed University), Wardha-442004, India
Corresponding Author : Madhur M Goyal
Department of Biochemistry
J. N. Medical College
Datta Meghe Institute of Medical Sciences (Deemed University), Wardha-442004, India
Tel: +919823585404
E-mail: [email protected]
Received August 01, 2012; Accepted August 29, 2012; Published August 31, 2012
Citation: Goyal MM, Meshram A, Basak A (2012) Hydroxyl Radical Generation by Mammalian Catalase: A Few Experimental Evidences. Biochem Pharmacol 1:103. doi:10.4172/2167-0501.1000103
Copyright: © 2012 Goyal MM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Catalase is well known antioxidant enzyme which protects cells from the toxic effects of hydrogen peroxide. Unlike peroxidase, it catalyses the dissociation of hydrogen peroxide directly into H2O and O2. Our results show that apart from the known catalytic action, mammalian catalase can generate Hydroxyl Radicals (HRs) in experimental conditions. At low concentrations of H2O2 (<20 μM), presence of bovine liver catalase (BLC) increased the generation of HRs in reaction mixture. Differential productions of HRs due to either presence of azide or urea induced structural changes of BLC suggest that the enzyme participate in the production of HRs. The possible character is unusual in contrast to the well established fact that catalase reduce the generation of reactive oxygen species in biological system. The work is being presented with discussion on experimental limitations we faced throughout the study.

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