Hypericum triquetrifolium Extracts Modulate IL-6, IL-10 and TNF-ÃÂ± Protein and mRNA Expression in LPS-Activated Human Peripheral Blood Mononuclear Cells and THP-1-Derived Macrophages
- Corresponding Author:
- Saad B
PhD, Qasemi Research Center
Al-Qasemi Academic College
Baqa El-Gharbia 30100, Israel
E-mail: [email protected]
Received April 03, 2016; Accepted April 28, 2016; Published May 02, 2016
Citation: Saad B, Embaslat WH, Abu-Farich B, Mahajna S, Azab M, et al. (2016) Hypericum triquetrifolium Extracts Modulate IL-6, IL-10 and TNF-α Protein and mRNA Expression in LPS-Activated Human Peripheral Blood Mononuclear Cells and THP-1-Derived Macrophages. Med Aromat Plants S3:004. doi:10.4172/2167-0412.S3-004
Copyright: © 2016 Saad B, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The aim of the present in vitro study was carried out to evaluate the anti-inflammatory activity of Hypericum triquetrifolium extracts (HT-extract) on lipopolyssacharide -stimulated human monocytic (THP-1) cells and human peripheral blood mononuclear cells (PBMNCs). The expression and production of pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin 6 (IL-6), as well as the anti-inflammatory cytokine interleukin 10 (IL-10) were evaluated by assessing the levels of proteins and mRNA’s of TNF-α, IL-6 and IL-10 in both cell types. Cells were exposed to 5 μg lipopolyssacharide (LPS) /ml in the absence and presence of increasing concentrations of 50% ethanol extracts from the aerial parts of Hypericum triquetrifolium. The anti-inflammatory efficacy experiments were performed with HT-extract concentrations up to 250 μg/ml that had no cytotoxic effects as assessed with MTT and LDH assays. HT-extract remarkably inhibited the expression and secretion of TNF-α and IL-6 at a concentration of 250 μg/ml. HT-extract remarkably elevated IL-10 secretion and mRNA levels at 125 μg/ml. Furthermore, HT-extract exhibited relatively high antioxidant activity (IC50 of 5 μg/ml) as measured with DPPH assay. These findings indicate that HT-extract probably exerts anti-inflammatory effects at both protein and gene expression levels of the anti-inflammatory as well of the pro-inflammatory cytokines in PBMNCs.