alexa Hyper-production of Alkaline Protease by Mutagenic Treatment of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged Fermentation | OMICS International | Abstract
ISSN: 1948-5948

Journal of Microbial & Biochemical Technology
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Research Article

Hyper-production of Alkaline Protease by Mutagenic Treatment of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged Fermentation

Sadia Javed1*, Munazzah Meraj2, Shazia Anwer Bukhari1, Rao Irfan3 and Saqib Mahmood4

1Department of Applied Chemistry & Biochemistry, Government College University, Faisalabad, Pakistan

2Department of Biochemistry, Peoples University of Medical and Health Sciences for Women, Nawabshah, Pakistan

3Department of Pharmaceutics, University of Sindh, Jamshoroo, Pakistan

4Department of Botany, Government College University, Faisalabad, Pakistan

*Corresponding Author:
Sadia Javed
Department of Applied Chemistry and Biochemistry
Government College University
Faisalabad, 38000, Pakistan
Tel: 92-333-6510755; 92-41-8505391
E-mail: [email protected], [email protected]

Received date: July 08, 2013; Accepted date: August 05, 2013; Published date: August 08, 2013

Citation: Javed S, Bukhari SA, Meraj M, Mahmood S (2013) Hyper-production of Alkaline Protease by Mutagenic Treatment of Bacillus subtilis M-9 using Agroindustrial Wastes in Submerged Fermentation. J Microb Biochem Technol 5:074-080. doi:10.4172/1948-5948.1000103

Copyright: © 2013 Javed S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited


Green chemistry technologies are the powerful tool for the management of environmental wastes challenges. Agro industrial residues are composed of complex polysaccharides that support the microbial growth for the production of useful products (enzymes, organic acids, drugs, etc.). Disposal and environment friendly management of these wastes has become a global priority. The aim of present investigation was to improve the alkaline protease yield by treating the parent Bacillus subtilis M-9 strain with different mutagens UV-irradiations, N-methyl-N-nitro- N-nitrosoguinidine (NTG), Ethidium bromide (EB), using agroindustrial wastes (banana stalk and corn stover) in submerged fermentation. Fifteen positive mutants were selected on skim milk agar plates for shake flask experiments. BSU-5 mutant strain showed 81.21± 3.24 PU/mL alkaline protease activity higher than parent strain (23.57 ± 1.19 PU/mL) in optimized fermentation medium. The fermentation profile like pH (9), temperature (45°C), inoculum size (2 mL), incubation time (24 hrs, and kinetic parameters such as u (h-1), Yp/s, Yp/x, Yx/s, qs, Qs, qp also confirmed the hyper proteolytic activity of alkaline protease produced from BSU-5 mutant strain over parent strain and other mutants. Finally, the BSU-5 mutant strain was immobilized by entrapping it in calcium alginate beads and agar. Alkaline protease production and stability of biocatalyst were investigated in both free and immobilized cells. It was concluded from the study, immobilized cells were more efficient for enzyme production then free cells when used repeatedly.


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