Journal of Plant Biochemistry & Physiology
Open Access

Abstract

Hypoglycemic and Hypolipidemic Potential of Nigella sativa L. Seed Extract in Streptozotocin (STZ)-Induced Diabetic Rats

Asaduzzaman M, Lutfun Nahar, Sohanur Rahman M, Mahadi Hasan, Anowara Khatun, Zinat Tamanna, Nazmul Huda, Fazley Rabbi M, Manobendro Nath Ray, Nur Islam M, Mobassirul Islam M, Maniruzzaman M and Mehfuz Alam

Diabetes mellitus (DM) is the most common metabolic disease worldwide. Multiple medications and side effects, the most significant and prevalent themes in diabetes mellitus ultimately determine novel directions and avenues in drug discovery. The objective of this study was to determine the effects of NS (Nigella sativa) L. Seed hydroalcholic Extract on hypoglycemic and hypolipidemic condition in Streptozotocin- induced diabetic rats. DM was induced by single intraperitoneal injection of freshly prepared Streptozotocin (STZ) (65 mg/kg body weight) solution. Mice were divided into six groups. This study was conducted for 28 days and blood glucose level was measured at 7 days interval. Hyperlipidemic was induced by mixture of cholesterol (1.5%) and cholic acid (0.5%) with diet of rats. At the end of the treatment, the blood glucose level and lipid profile was measured by using commercial kits. It was observed that NS (Nigella sativa) has a potential hypoglycemic effect as it significantly (p<0.001) decrease blood glucose level compared to diabetic group. The SGPT, SGOT and CRP were also decreased significantly (p<0.05). Therefore NS (Nigella sativa) might be effective against liver malfunction. An indicative antilipidemic effect was also observed as TC, TG, LDL, VLDL showed significant (p<0.05) decrease whereas HDL showed significant increase (p<0.001) by NS (Nigella sativa) treatment compared to diabetic group. These results showed that hydroalcholic extract of NS (Nigella sativa) at low doses has hypoglycemic effect and as well as lipid profile in diabetic subjects. Nigella sativa is a potential protective natural agent against atherosclerosis, hepatoprotective and cardiovascular complication in diabetes rats.