alexa Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia | OMICS International
ISSN: 2161-0525

Journal of Environmental & Analytical Toxicology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia

Metasebia Aklilu1*, Tesfaye Sisay2, Genene Tefera3 and Belay Tekalign4

1Addis Ababa University, College of Veterinary Medicine and Agriculture, Debrezeite, Ethiopia

2Addis Ababa University, Institute Biotechnology, Department of Health Biotechnology, Addis Ababa, Ethiopia

3Institute of Biodiversity center, Addis Ababa, Ethiopia

4Mereced University, California, USA

*Corresponding Author:
Metasebia Aklilu
Department of Microbiology
Immunology and Veterinary Public Health
College of Veterinary Medicine and Agriculture
Addis Ababa University
Addis Ababa, Ethiopia
E-mail: [email protected]

Received Date: July 25, 2013; Accepted Date: August 29, 2013; Published Date: September 02, 2013

Citation: Aklilu M, Sisay T, Tefera G, Tekalign B (2013) Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debre Birhan Town, Ethiopia. J Environ Anal Toxicol 3:188. doi: 10.4172/2161-0525.1000188

Copyright: © 2013 Aklilu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Background: Infectious diarrhea is the most significant cause of morbidity and mortality in neonatal dairy sheep throughout the world. This is the first study conducted on isolation and biotyping of E. coli from lamb diarrhea in and around Debre Birhan. The present study was undertaken with the objective of isolation, omnilog characterization and biotyping of E. coli isolates from faecal samples around Debrebrhan.
Methods: A cross-sectional study was conducted from October, 2012 to April, 2013. The study focused on lambs less than three months of age showing clinical symptoms of diarrhea. Standard cultural and Omnilog tests were done to identify E. coli species and biotypes; descriptive statistics and Chi-square were used to analyze the collected data.
Results: From a total of 100 diarrheic lambs examined, 84% were found to be positive for E. coli. On the basis of fermentation reactions of sugars viz. dulcitol, raffinose, rhamnose, salicin, starch and sucrose, 70 isolates utilized one or more sugar while 14 isolates failed to utilize any of the sugars. 70 E. coli isolates were biotyped into 15 different combinations. Association between different age groups and occurrence of lamb diarrhea caused by E. coli strains showed a significant association (p < 0.05). From the questionnaire findings, 60% of all the health problems in lambs were due to diarrhea where young sheep were more affected.
Conclusion: pathogenic E. coli has the potential to cause sheep diarrhea. The distribution of the E. coli isolates into different biotypes indicates the diverse nature of the organism. Therefore, further detailed study should be carried out to understand the role of E. coli in lamb diarrhea and identify the virulent strains involved.

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version