alexa Identification and Differentiation of Highly Diverse Campylobacter concisus Strains using the MALDI Biotyper
ISSN: 2327-5073

Clinical Microbiology: Open Access
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Identification and Differentiation of Highly Diverse Campylobacter concisus Strains using the MALDI Biotyper

Hans Linde Nielsen1*, Mette Mølvadgaard1, Henrik Nielsen2,3 and Markus Kostrzewa4

1Department of Clinical Microbiology, Aalborg University Hospital, Aalborg, Denmark

2Department of Infectious Diseases, Aalborg University Hospital, Aalborg, Denmark

3Department of Clinical Medicine, Aalborg University, Aalborg Øst, Denmark

4Bruker Daltonik GmbH, Bremen, Germany

*Corresponding Author:
Hans Linde Nielsen
Department of Clinical Microbiology
Aalborg University Hospital
Hobrovej 18-22, DK-9000 Aalborg, Denmark
Tel: +45-97-66-54-23
Fax: +45-97-66-54-25
E-mail: [email protected]

Received Date: December 11, 2015; Accepted Date: January 14, 2016; Published Date: January 21, 2016

Citation: Nielsen HL, Mølvadgaard M, Nielsen H, Kostrzewa M (2016) Identification and Differentiation of Highly Diverse Campylobacter concisus Strains using the MALDI Biotyper. Clin Microbial 5:230. doi: 10.4172/2327-5073.1000230

Copyright: © 2016 Nielsen HL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

In Danish diarrheic stool samples, the emerging enteric pathogen Campylobacter concisus has an incidence at the level of Campylobacter jejuni. Nevertheless, C. concisus has been neglected in many clinical laboratories due to labour intensive isolation procedures as well as time-consuming PCR-based methods for identification. Fourteen isolates of C. concisus were characterised in the MALDI-TOF mass spectrometer and analysed using the MALDI Biotyper and the ClinProTools 3.0 software. The data show that MALDI-TOF MS, as previously described for other Campylobacter species, can give a rapid identification of C. concisus. Interestingly, the isolates showed a remarkable diversity based upon their mass spectra when compared by visual and computational analysis.

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