alexa Identification and Quantification of Paralytic Shellfis
ISSN: 2380-2391

Journal of Environmental Analytical Chemistry
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Research Article

Identification and Quantification of Paralytic Shellfish Toxins in Puget Sound Marine Organisms

Strawn ML1, Lunsford ET2, Larsen LE1, Moran MM1, Allbritton V1, Ferrer RP2 and Pierce KM1*

1Department of Chemistry and Biochemistry, Seattle Pacific University, Seattle, WA 98119, USA

2Department of Biology, Seattle Pacific University, Seattle, WA 98119, USA

*Corresponding Author:
Pierce KM
Department of Chemistry and Biochemistry
Seattle Pacific University
3307 Third Avenue West, Suite 205
Seattle, WA 98119, USA
Tel: 2062812102
E-mail: [email protected]

Received date: July 07, 2015; Accepted date: July 10, 2015; Published date: July 12, 2015

Citation: Strawn ML, Lunsford ET, Larsen LE, Moran MM, Allbritton V, et al. (2015) Identification and Quantification of Paralytic Shellfish Toxins in Puget Sound Marine Organisms. J Environ Anal Chem 2:148. doi: 10.4172/2380-2391.1000148

Copyright: © 2015 Strawn ML, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Saxitoxin and its structural analog neosaxitoxin were identified and quantified in mussels and sea stars that were collected from the Puget Sound region during harmful algal blooms. The analysis was based on high performance liquid chromatography with fluorescence detection and pre-column oxidation. Quantitative analytical results revealed that saxitoxin and neosaxitoxin were present in some of the Puget Sound samples well above toxic levels (up to 265 μg saxitoxin/100 g tissue and 111 μg neosaxitoxin/100 g tissue). For all of the samples of interest, paralytic shellfish toxins other than saxitoxin and neosaxitoxin were semi-quantified and a total toxicity value was estimated for each sample. The samples ranged from nontoxic to very toxic (up to 3687 μg saxitoxin-equivalents/100 g tissue).

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