Identification and Typing of Aeromonas Hydrophila through 16S rDNA-PCR Fingerprinting
- *Corresponding Author:
- Agniswar Sarkar
Department of Biotechnology
The University of Burdwan
Golapbag more, Burdwan
West Bengal- 713 104, India
E-mail: [email protected]
Received Date: May 31, 2012; Accepted Date: July 26, 2012; Published Date: August 10, 2012
Citation: Sarkar A, Saha M, Roy P (2012) Identification and Typing of Aeromonas Hydrophila through 16S rDNA-PCR Fingerprinting. J Aquacult Res Dev 3:146. doi:10.4172/2155-9546.1000146
Copyright: © 2012 Sarkar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
16S rRNA is a conserved biomolecule within a cell. Sequencing data was analyzed and examined by sequencing the corresponding 16s rDNA regions of isolates from different sources. Specific culture media and different biochemical tests primarily confirmed isolates as Aeromonas hydrophila; those were designed and tested following PCR assays and identified by 16S rDNA gene sequence analysis. To automate the method few online and offline computational tools have been used. The method has proven useful for identification of Aeromonas species. Our results emphasize the need to take into account the intragenomic diversity of the 16S rRNA gene. Different software and programme used in this study are freely available online in different website.