alexa Identification of Endophytic Bacteria and their Characterization as Biocontrol Agents against Tomato Southern Blight Disease
ISSN: 2471-9315

Applied Microbiology: Open Access
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Identification of Endophytic Bacteria and their Characterization as Biocontrol Agents against Tomato Southern Blight Disease

Claudia Mónica Ribaudo*, Daniela Soledad Riva, Juan Ignacio Gori, José Ignacio Zaballa and Catalina Molina

Facultad de Agronomía, Universidad de Buenos Aires, Cátedra de Bioquímica, Avenida San Martín 4453, C1417DSE Buenos Aires, Argentina

Corresponding Author:
Claudia Mónica Ribaudo
Facultad de Agronomía, Universidad de Buenos Aires
Cátedra de Bioquímica, Avenida San Martín 4453, C1417DSE Buenos Aires, Argentina
Tel: +54 11 4524 8087
E-mail: [email protected]

Received Date: August 24, 2016; Accepted Date: October 04, 2016; Published Date: October 05, 2016

Citation: Ribaudo CM, Riva DS, Gori JI, Zaballa JI, Molina C (2016) Identification of Endophytic Bacteria and their Characterization as Biocontrol Agents against Tomato Southern Blight Disease. Appli Micro Open Access 2:123. doi:10.4172/2471-9315.1000123

Copyright: © 2016 Ribaudo CM, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Objectives: The purpose of the present study was to characterize the endophytic bacterium PAC BNM0522 and to evaluate its role in the ethylene signaling pathway in the development of southern blight disease (caused by the necrotrophic fungus Sclerotium rolfsii) in tomato plants. Methods: In this study, biochemical tests, 16S rRNA gene sequencing, and amplification of the ACCD gene (acdS) were performed on the isolate obtained from pruning compost. This isolate was also screened for antibiotic production and competition for nutrients and evaluated its role in the ethylene signaling pathway by analysis of the expression of genes involved in ethylene biosynthesis by PCR and ethylene content by GC. Results: The analysis of the 16S rRNA gene partial sequence and biochemical test placed this bacterium within the genus Pseudomonas and revealed that it belongs to the group of PGPR with multiple plant growth-promoting traits. This rhizobacterium was able to promote host growth through direct antagonism to the fungal pathogen. The in vivo experiments showed that PAC BNM0522 inoculation controlled the disease caused by S. rolfsii. The ethylene level was enhanced in tomato plants infected with the fungus and decreased by inoculation of the rhizobacterium. The expression of the Sl-ACS2 isoform was induced by the presence of the pathogen and strongly repressed in PAC BNM0522-primed plants. The accumulation of the Sl-ACO1 messenger was down-regulated in deaminaseproducing PAC BNM0522-inoculated plants. Conclusion: Our results indicate that ACCD-containing PAC BNM0522 generated protection of tomato plants under pathogenic stress and alleviated ethylene-induced damage, by regulating the expression of genes involved in the ethylene pathway. PAC BNM0522 may be used as an alternative ecological approach to control soil-borne fungi.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords