alexa Identification of Host Plant Resistant to Dolichos Yellow Mosaic Virus (DYMV) in Dolichos Bean (Lablab Purpureus) | OMICS International | Abstract
ISSN: 2157-7471

Journal of Plant Pathology & Microbiology
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Research Article

Identification of Host Plant Resistant to Dolichos Yellow Mosaic Virus (DYMV) in Dolichos Bean (Lablab Purpureus)

PK Singh1, Ashish Kumar1,2*, N Rai1 and DV Singh3

1Division of Crop Improvement, Indian Institute of Vegetable Research (IIVR), P.O. - BHU, Varanasi, Uttar Pradesh 221 005, India

2Division of Crop Sciences, Central Research Institute for Dryland Agriculture (CRIDA), Santosh Nagar, Saidabad, Hyderabad, Andhra Pradesh 500 059, India

3Department of Botany, Udai Pratap Autonomous College, Varanasi, Uttar Pradesh 221 001, India

*Corresponding Author:
Ashish Kumar
Division of Crop Sciences
Central Research Institute for Dryland Agriculture (CRIDA)
Santosh Nagar, Saidabad, Hyderabad
Andhra Pradesh 500 059, India
Tel: +91-949 386 5018
E-mail: [email protected]

Received date: August 02, 2012; Accepted date: September 10, 2012; Published date: September 15, 2012

Citation: Singh PK, Ashish Kumar, Rai N, Singh DV (2012) Identification of Host Plant Resistant to Dolichos Yellow Mosaic Virus (DYMV) in Dolichos Bean (Lablab Purpureus). J Plant Pathol Microb 3:130. doi:10.4172/2157-7471.1000130

Copyright: © 2012 Singh PK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Three hundred Dolichos bean (Lablab purpureus) genotypes were screened against Dolichos Yellow Mosaic Virus (DYMV) disease. Initial screening was done under field conditions, where disease incidence was calculated for each genotype. Subsequently, selfed progenies of 34 symptomless lines were challenged by sap inoculation under field conditions, out of which only three genotypes, viz. VRSEM-894, VRSEM-887 and VRSEM-860 did not show any symptoms. Using root stalk of susceptible genotype (Ankur Goldy), these three putative symptomless genotypes were further challenged by grafting. The resistant reactions of VRSEM-894, VRSEM-887 and VRSEM-860 were confirmed, as even after 60 days of successful grafting, no viral symptom appeared on all the grafted plants of these genotypes. When subjected to PCR amplification with DYMV coat protein gene specific primer, these three symptomless genotypes did not show any amplification, suggesting that there was no infection of Dolichos Yellow Mosaic Virus in those genotypes.

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