alexa Identification of Lactobacillus Fermentum Strains with Potential against Colorectal Cancer by Characterizing Short Chain Fatty Acids Production, Anti-Proliferative Activity and Survival in an Intestinal Fluid: In Vitro Analysis
ISSN: 1948-593X

Journal of Bioanalysis & Biomedicine
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Identification of Lactobacillus Fermentum Strains with Potential against Colorectal Cancer by Characterizing Short Chain Fatty Acids Production, Anti-Proliferative Activity and Survival in an Intestinal Fluid: In Vitro Analysis

Imen Kahouli1,2, Meenakshi Malhotra1, Catherine Tomaro-Duchesneau1, Laëtitia Sonia Rodes1, Moulay A Aloui-Jamali3,4 and Satya Prakash1,2*

1Biomedical Technology and Cell Therapy Research Laboratory-Departments of Biomedical Engineering, Physiology, and Artificial Cells and Organs Research Center, Faculty of Medicine, McGill University, Canada

2Department of Experimental Medicine, Faculty of Medicine, McGill University, Canada

3Departments of Medicine and Oncology, Faculty of Medicine, McGill University, Canada

4Lady Davis Institute for Medical Research and Segal Cancer Centre, Sir Mortimer B. Davis-Jewish General Hospital, Canada

*Corresponding Author:
Satya Prakash
Department of Experimental Medicine
Faculty of Medicine, McGill University, Canada
Tel: +1-514-398-3676
Fax: +1-514-398-7461
Email: [email protected]

Received Date: May 28, 2015 Accepted Date: July 06, 2015 Published Date: July 09, 2015

Citation: Kahouli I, Malhotra M, Tomaro-Duchesneau C, Rodes LS, Aloui-Jamali MA, et al. (2015) Identification of Lactobacillus Fermentum Strains with Potential against Colorectal Cancer by Characterizing Short Chain Fatty Acids Production, Anti-Proliferative Activity and Survival in an Intestinal Fluid: In Vitro Analysis. J Bioanal Biomed 7:104-115. doi:10.4172/1948-593X.1000132

Copyright: © 2015 Kahouli I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

The use of probiotics as preventive agents in colorectal cancer (CRC) was widely suggested in many clinical and pre-clinical studies and often linked to affecting short chain fatty acids (SCFA) in the gut. However, the understanding of fatty-acid-producing activity of certain probiotics and their preventive and anti-cancer potential remains incomplete. Here, L. fermentum strains were investigated for a number of features that make them good candidates for use in CRC treatments. Using cell free extracts, L. fermentum NCIMB -5221, -2797 and -8829 were first compared based on SCFAs production and anti-proliferative activity against Caco-2 colon cancer cells. The corresponding SCFAs synthetic formulations, similar to the ones produced by the bacteria, were prepared then compared with the latter to determine the role an efficacy of naturally produced SCFAs in inhibiting the proliferation of colon cancer cells. Later, the bioactivity and stability of L. fermentum bacteria in a simulated intestinal fluid (SIF) were determined. Results showed that L. fermentum NCIMB -5221 and -8829 were the most potent in producing SCFAs, in particular, acetic (192.3 ± 4 mg/L minimum), propionic (69.2 ± 1.6 mg/L minimum), and butyric (35.4 ± 2.9 mg/L minimum) acids and were observed to better inhibit the growth of Caco-2 cells (53.4 ± 1.6%, 72 h, p=0.021) in comparison with L. acidophilus ATCC 314. They also showed resistance to SIF (16.3 ± 1.9% minimum, 72 h, p=0.006) and produced SCFAs in SIF at concentrations high enough to significantly inhibit Caco-2 proliferation (74.73 ± 2.1%, 72 h). Potentially, based on characteristics related to bacterial cell survival and SCFA production, and anti-proliferative activity, certain L. fermentum could be considered as biotherapeutic agent against CRC.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri, Food, Aqua and Veterinary Science Journals

Dr. Krish

[email protected]

1-702-714-7001 Extn: 9040

Clinical and Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemical Engineering and Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001 Extn: 9040

Earth & Environmental Sciences

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

General Science and Health care Journals

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics and Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001 Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Informatics Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Material Sciences Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Mathematics and Physics Journals

Jim Willison

[email protected]

1-702-714-7001 Extn: 9042

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001 Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

John Behannon

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001 Extn: 9042

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords