Identification of Milk Protein Polymorphism in Indian Goats by 2D Gel Electrophoresis
- *Corresponding Author:
- Promod Kumar Rout
Genetics and Breeding Division
Central Institute for Research on Goats
Makhdoom, Farah, Mathura-281122, India
E-mail: [email protected]
Received Date: October 09, 2012; Accepted Date: December 31, 2012; Published Date: January 02, 2013
Citation: Kumar A, Rout PK, Mohanty BP (2013) Identification of Milk Protein Polymorphism in Indian Goats by 2D Gel Electrophoresis. J Proteomics Bioinform 6: 001-004. doi: 10.4172/jpb.1000252
Copyright: © 2013 Kumar A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Analysis of casein and whey protein was carried out in six Indian goats by both SDS-PAGE and 2-DE analysis. The variation was observed mainly in αs1, αs2, β, and κ-casein and β-lacto globulin locus in these breeds. Proteome analysis showed the presence of high number of spots in αs1-casein, and κ-casein showed highest number of spots in all the goat breeds. The gels were showing remarkably both variability and similarity suggesting that the heterogeneity in protein forms in individual milk samples exist, and milk protein represents a common pattern of post-translational modification.