alexa Identification of Proteins in Tissue Fluids of the sea mussel Isognomonalatus | OMICS International
ISSN: 2157-7064

Journal of Chromatography & Separation Techniques
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Research Article

Identification of Proteins in Tissue Fluids of the sea mussel Isognomonalatus

Angel Alberto Justiz Vaillant1*, Suzette Curtello2, Monica Smikle3, Brian Wisdom4, Wayne Mohammed1, Sehlule Vuma1, Geeta Kurhade5 , Norma McFarlane-Anderson2, Chalapathi R1, Shivnarine Kissoon1

1Pathology and Microbiology Unit, Department of Para-Clinical Sciences,The University of the West Indies, St. Augustine, Trinidad and Tobago

2Department of Basic Medical Sciences, The University of the West Indies, Mona, Jamaica

3Department of Microbiology, The University Hospital of West Indies, Mona, Jamaica

4School of Biology and Biochemistry, Medical Biology Centre, The Queen’s University of Belfast, Belfast, Ireland

5Physiology Section, Department of Pre-clinical Sciences, The University of the West Indies, St Augustine, Trinidad and Tobago

*Corresponding Author:
Angel Justiz Vaillant
Department of Para-Clinical Sciences
The University of the West Indies
St. Augustine, Trinidad and Tobago
Tel: +868-736-0440
Fax: +868-663-3797
E-mail: [email protected]

Received Date: August 29, 2013; Accepted Date: September 29, 2013; Published Date: October 04, 2013

Citation: Vaillant AAJ, Farlane-Anderson M, Smikle M, Wisdom B, Mohammed W, et al. (2013) Identification of Proteins in Tissue Fluids of the sea mussel Isognomonalatus. J Chromatograph Separat Techniq 4:203. doi: 10.4172/2157-7064.1000203

Copyright: © 2013 Vaillant AAJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of this preliminary study was to investigate the presence of proteins in the tissue fluids of the sea mussel Isognomonalatus. Protein extraction was done by the chloroform-cold ethanol technique. Immunization for production of antibody to be used as reagents in Western blotting, assessment of the protein concentration by the Bradford method, protein characterization by native polyacrylamide gel electrophoresis (PAGE) were also performed as a part of the methodology of this study. The results showed a protein content of 65 mg/ml in tissue fluids and a protein band approximately of 220 kDa in PAGE that was further confirmed by the Western blotting. Future work should investigate the structure and function of the proteins separated from the tissue fluids and we considered it as a limitation of this investigation. The sea bivalve literature is scanty. However the limitation of this work we still can conclude that there are high molecular weight proteins in large concentrations in tissue fluids of the sea mussel Isognomomalatus.

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