alexa Identification of Thyrotropin-Releasing Hormone (TRH)-D
ISSN: 2161-1122

Dentistry
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Identification of Thyrotropin-Releasing Hormone (TRH)-Degrading Enzyme as a Biomarker for Dental Pulp Tissue

Tsubasa Yamamoto1,2, Masashi Murakami1, Ryo Ishizaka1,3, Koichiro Iohara1, Kenichi Kurita2 and Misako Nakashima1*

1Center of Advanced Medicine for Dental and Oral Diseases, National Center for Geriatrics and Gerontology, Research Institute, Obu 474-8511, Japan

2 Department of Oral and Maxillofacial Surgery, School of Dentistry, Aichigakuin University, Nagoya 464-8651, Japan

3 Department of Pediatric Dentistry, School of Dentistry, Aichigakuin University, Nagoya 464-8651, Japan

*Corresponding Author:
Misako Nakashima Department of Dental Regenerative Medicine
Center of Advanced Medicine for Dental and Oral Diseases
National Center for Geriatrics and Gerontology
Research Institute, 35 Gengo, Morioka
Obu, Aichi 474-8511, Japan
Tel: 81 562 44 5651 (ext 5065)
Fax: 81 562 46 8684
E-mail: [email protected]

Received date October 20, 2011; Accepted date December 12, 2011; Published date January 14, 2012

Citation: Yamamoto T, Murakami M, Ishizaka R, Iohara K, Kurita K, et al. (2012) Identification of Thyrotropin-Releasing Hormone (TRH)-Degrading Enzyme as a Biomarker for Dental Pulp Tissue. Dentistry 2:114. doi:10.4172/2161-1122.1000114

Copyright: © 2012 Yamamoto T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Regeneration of dental pulp after pulpectomy was accomplished recently by autologous transplantation of dental pulp stem/ progenitor cells into the root canal. The identical patterns of qualitative and quantitative protein and mRNA expression in the regenerated pulp and normal pulp demonstrated complete pulp regeneration. The lack of tissue specific markers in the dental pulp is a major challenge in dental regenerative medicine. In order to identify specific markers in dental pulp we undertook a comparison of the gene expression profile of the dental pulp with that of periodontal ligament and gingiva. This systematic investigation identified thyrotropinreleasing hormone (TRH)-degrading enzyme (DE) as a marker of dental pulp. Expression of TRH-DE mRNA in human dental pulp was higher than that in any other tissue except brain as analyzed by real time RT-PCR. Induction of neural cells enhanced the expression of TRH-DE mRNA in dental pulp stem/progenitor cells (CD105+ and CD31- side population (SP) cells) in vitro. Immunohistochemical and in situ hybridization analyses demonstrated that TRH-DE in the neuronal processes in dental pulp. In canine pulp cells, TRH down-regulated TRH-DE mRNA expression, while neuropeptide Y up-regulated it, suggesting that TRH-DE has functional role in the neuropeptide signaling in dental pulp tissue. It is noteworthy that TRH-DE mRNA was expressed in the regenerated pulp 28 days after transplantation of CD31- SP cells into root canals after pulpectomy. These results demonstrate the utility of TRH-DE as a novel dental pulp biomarker during regeneration of pulp.

Keywords

Share This Page

Additional Info

Loading
Loading Please wait..
 
Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2017-18
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

 
© 2008-2017 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version
adwords