Identification of Two Novel Regulatory Elements in the IL2RA Gene Locus
Received Date: Apr 30, 2018 / Accepted Date: May 03, 2018 / Published Date: May 09, 2018
Regulatory T-cells (Tregs) express the high-affinity chain of the interleukin 2 (IL-2) receptor, CD25, encoded by the IL2RA gene. IL2RA is one of the most extensively characterized genes regarding its regulatory regions and their functional links to cell surface receptors and their associated signal transduction cascade in the context of the antigen-dependent activation of mature CD4+ T lymphocyte. However, converging evidences strongly suggested that they were some missing pieces in this already complex puzzle made of 6 well-characterized regulatory regions. Here, by combining principally in silico genomic footprinting approach and meta-analysis of several ChIP-seq studies, we identified and characterized 2 new putative CD28-responsive elements. We show that a recently-characterized intronic enhancer at +11 kb harbors a functional CREB site. Further, we evidence a repressor element consisting of two GAAA repeats located 5’-most of a previously identified enhancer 4 kb upstream of the IL2RA gene. Massspectrometry analyses revealed Poly ADP-ribose polymerase 1 (PARP-1) as part of the complexes binding this element. Altogether, our observations extend our understanding of the molecular basis of the multiple options offered by such a complex organization in term of T cell responses.
Keywords: IL2RA; Enhancer; Regulatory T-cell; Gene regulation; CREB; PARP-1; EMSA
Citation: Rosa F, Rameil P, Algarte-Genin M, Bedotto M, Ferrier P, et al. (2018) Identification of Two Novel Regulatory Elements in the IL2RA Gene Locus. J Pulm Respir Med 8: 458. Doi: 10.4172/2161-105X.1000458
Copyright: © 2018 Rosa F, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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