alexa Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF -Special Reference to Human Brain Ganglioside Molecular Species
ISSN: 2153-0637

Journal of Glycomics & Lipidomics
Open Access

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Special Issue Article

Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF -Special Reference to Human Brain Ganglioside Molecular Species

Tania Valdes-Gonzalez1*, Naoko Goto-Inoue2, Takahiro Hayasaka2, Hironobu Ishiyama1, Mitsutoshi Setou2 and Takao Taki3*

1Third Institute of New Drug Discovery, Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan

2Department of Molecular Anatomy, Molecular Imaging, Frontier Research Center; Hamamatsu University School of Medicine, 1-20-1 Handayama, Higashi-ku Hamamatsu, Shizuoka 431-3192, Japan

3Institute of Biomedical Innovation, Otsuka Pharmaceutical Co. Ltd., 463-10 Kagasuno, Kawauchi-cho, Tokushima 771-0192, Japan

*Corresponding Author:
Tania Valdes-Gonzalez
Finlay Institute
Av 27 No:19805 esq 198 LA Coronela
La Lisa 11600 Habana, Cuba
Tel: 208 6086
Fax 2086075
E-mail: [email protected]

Takao Taki
Institute of Biomedical Innovation
Otsuka Pharmaceutical Co. Ltd. 463-10 Kagasuno
Kawauchi-cho, Tokushima 771-0192, Japan
Tel: +81-88-665- 2126
Fax: +81-88-665-6976
E-mail: [email protected]

Received date: April 20, 2011; Accepted date: July 15, 2011; Published date: July 22, 2011

Citation: Valdes-Gonzalez T, Goto-Inoue N, Hayasaka T, Ishiyama H, Setou M, et al. (2012) Imaging Technology of Complex Lipid Molecular Species by a Combination of TLC-Blot and MALDI-TOF –Special Reference to Human Brain Ganglioside Molecular Species. J Glycomics Lipidomics S2:004. doi: 10.4172/2153-0637.S2-004

Copyright: © 2012 Valdes-Gonzalez T, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Glycosphingolipids and phospholipids from white matter of human hippocampus, were analyzed by a sequential procedure of two-dimensional TLC (2d-TLC), transference of separated lipids to a PVDF membrane by a TLC-Blot equipment and Mass spectrometry (MS) analysis with an ion-trap type MALDI-TOF equipment. The method is simple and quick; very small amount of sample (0.1 mg of brain tissue) is enough to analyze all lipid components. The 2d-TLC provided excellent separation and the MS analyses allowed identifying the characteristic profile of molecular species for individual glycolipids and phospholipids. The results of MS analyses on gangliosides showed that di- and tri-sialogangliosides are richer in d20:1 sphingosine-containing ceramide than monosialogangliosides, suggesting the presence of sialylation selection after GM1 gangliosides. Then we analyzed ganglioside molecular species obtained from different brain regions by using MS imaging technology. The MS images of individual gangliosides provided clear visual profiles in terms of molecular species distribution. The imaging profiles were region dependent and also indicated that the sialyltransferase toward GM1 ganglioside prefers to select d20:1 sphingosine containing molecule. This technology provides visual characterization of individual phospholipid and glycosphingolipid molecular species and informs us about the metabolic characterization of target tissue, opening a new gate for colorful lipidomics research.

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