alexa Immobilization of Gluconobacter oxydans by Entrapment in Porous Chitosan Sponge
ISSN: 2155-9821

Journal of Bioprocessing & Biotechniques
Open Access

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Research Article

Immobilization of Gluconobacter oxydans by Entrapment in Porous Chitosan Sponge

Cunxun Wang, Kefeng Ni, Xu Zhou, Dongzhi Wei and Yuhong Ren*
State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, China
Corresponding Author : Yuhong Ren
State Key Laboratory of Bioreactor Engineering
New World Institute of Biotechnology
East China University of Science and Technology
Shanghai 200237, China
Tel: +86-2164252163
Fax: +86-2164250068
Email: [email protected]
Received August 08, 2013; Accepted August 22, 2013; Published August 30, 2013
Citation: Wang C, Ni K, Zhou X, Wei D, Ren Y (2013) Immobilization of Gluconobacter oxydans by Entrapment in Porous Chitosan Sponge. J Bioprocess Biotech 3:132. doi:10.4172/2155-9821.1000132.
Copyright: © 2013 Wang C, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
 

Abstract

The porous chitosan sponge was prepared using NaHCO3 as the porogen and used to immobilize Gluconobacter oxydans. Under the optimum conditions, the activity recovery of the immobilized cells reached 92%. The morphology characterization of the immobilized cells revealed that the cells were attached to the surface of the pores (100-400μm) which were well distributed in the chitosan sponge. The valuation of cell activity showed that the immobilized cells displayed enhanced pH and thermal stability compared to free cells. Furthermore, the immobilized cells retained 74% of its origin activity after 12 repeated reaction cycles separated by filtration.

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