Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNÃŽÂ³+LPS) or Alternatively Activated M(IL-4) Macrophages
|Andra Banete, Paulina Achita, Katherine Harding, Rylend Mulder and Sameh Basta*|
|Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada|
|Corresponding Author :||Samesh Basta
Department of Biomedical and
Molecular Sciences, Queen’s University
Kingston, Ontario, Canada
E-mail: [email protected]
|Received February 19, 2015, Accepted April 08, 2015, Published April 15, 2015|
|Citation: Banete A, Achita P, Harding K, Mulder R, Basta S (2015) Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(Ifnγ+Lps) or Alternatively Activated M(Il-4) Macrophages. J Clin Cell Immunol 6:318. doi: 10.4172/2155-9899.1000318|
|Copyright: © 2015 Basta S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Objective: Macrophages (MÃÂ•) represent a link between the innate and adaptive arms of the immune system. Generally, MÃÂ• are classified into two major subsets after stimulation; either ascribed classically (M1), or more specifically M(IFNγ+LPS) based on the activating condition, or alternatively (M2), or more specifically M(IL-4) activated cells. The purpose of the study was to evaluate an immortalized murine MÃÂ• cell line (BMA) as an in vitro model for MÃÂ• polarization into M(IFNγ+LPS) and M(IL-4) phenotypes to facilitate the progress in this exciting research field.
Methods: The BMA cell line was stimulated with either IFNγ and LPS or IL-4 to induce cellular polarization. The cells were characterized using multi-parameter analyses employing phenotypic and functional assays, and compared to bone-marrow derived macrophages (BMDM).
Results: The BMA cell line was found to differentiate into either M(IFNγ+LPS) MÃÂ•, characterized by production of inflammatory cytokines and up-regulation of inducible nitric oxide synthase (iNOS) or M(IL-4) cells with high Arginase-1 activity. Furthermore, polarized BMA cells were found to have a differential expression of cell surface markers.
Conclusion: These findings demonstrate that the BMA cell line can be polarized into M(IFNγ+LPS)/M(IL-4) phenotypes, and can therefore be used as a model for in vitro MÃÂ• polarization reducing the need for primary MÃÂ• isolation when investigating biological phenomena related to their polarization.