alexa Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNγ+LPS) or Alternatively Activated M(IL-4) Macrophages | Abstract
ISSN: 2155-9899

Journal of Clinical & Cellular Immunology
Open Access

Like us on:
OMICS International organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations

700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Review Article

Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(IFNγ+LPS) or Alternatively Activated M(IL-4) Macrophages

Andra Banete, Paulina Achita, Katherine Harding, Rylend Mulder and Sameh Basta*
Department of Biomedical and Molecular Sciences, Queen’s University, Kingston, Canada
Corresponding Author : Samesh Basta
Department of Biomedical and
Molecular Sciences, Queen’s University
Kingston, Ontario, Canada
Tel: 613-533-6648
E-mail: [email protected]
Received February 19, 2015, Accepted April 08, 2015, Published April 15, 2015
Citation: Banete A, Achita P, Harding K, Mulder R, Basta S (2015) Immortalized Murine Macrophage Cell Line as a Model for Macrophage Polarization into Classically Activated M(Ifnγ+Lps) or Alternatively Activated M(Il-4) Macrophages. J Clin Cell Immunol 6:318. doi: 10.4172/2155-9899.1000318
Copyright: © 2015 Basta S. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Abstract

Objective: Macrophages (Mϕ) represent a link between the innate and adaptive arms of the immune system. Generally, Mϕ are classified into two major subsets after stimulation; either ascribed classically (M1), or more specifically M(IFNγ+LPS) based on the activating condition, or alternatively (M2), or more specifically M(IL-4) activated cells. The purpose of the study was to evaluate an immortalized murine Mϕ cell line (BMA) as an in vitro model for Mϕ polarization into M(IFNγ+LPS) and M(IL-4) phenotypes to facilitate the progress in this exciting research field.

Methods: The BMA cell line was stimulated with either IFNγ and LPS or IL-4 to induce cellular polarization. The cells were characterized using multi-parameter analyses employing phenotypic and functional assays, and compared to bone-marrow derived macrophages (BMDM).

Results: The BMA cell line was found to differentiate into either M(IFNγ+LPS) Mϕ, characterized by production of inflammatory cytokines and up-regulation of inducible nitric oxide synthase (iNOS) or M(IL-4) cells with high Arginase-1 activity. Furthermore, polarized BMA cells were found to have a differential expression of cell surface markers.

Conclusion: These findings demonstrate that the BMA cell line can be polarized into M(IFNγ+LPS)/M(IL-4) phenotypes, and can therefore be used as a model for in vitro Mϕ polarization reducing the need for primary Mϕ isolation when investigating biological phenomena related to their polarization.

Keywords

Peer Reviewed Journals
 
Make the best use of Scientific Research and information from our 700 + peer reviewed, Open Access Journals
International Conferences 2018-19
 
Meet Inspiring Speakers and Experts at our 3000+ Global Annual Meetings

Contact Us

Agri & Aquaculture Journals

Dr. Krish

[email protected]

1-702-714-7001Extn: 9040

Biochemistry Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Business & Management Journals

Ronald

[email protected]

1-702-714-7001Extn: 9042

Chemistry Journals

Gabriel Shaw

[email protected]

1-702-714-7001Extn: 9040

Clinical Journals

Datta A

[email protected]

1-702-714-7001Extn: 9037

Engineering Journals

James Franklin

[email protected]

1-702-714-7001Extn: 9042

Food & Nutrition Journals

Katie Wilson

[email protected]

1-702-714-7001Extn: 9042

General Science

Andrea Jason

[email protected]

1-702-714-7001Extn: 9043

Genetics & Molecular Biology Journals

Anna Melissa

[email protected]

1-702-714-7001Extn: 9006

Immunology & Microbiology Journals

David Gorantl

[email protected]

1-702-714-7001Extn: 9014

Materials Science Journals

Rachle Green

[email protected]

1-702-714-7001Extn: 9039

Nursing & Health Care Journals

Stephanie Skinner

[email protected]

1-702-714-7001Extn: 9039

Medical Journals

Nimmi Anna

[email protected]

1-702-714-7001Extn: 9038

Neuroscience & Psychology Journals

Nathan T

[email protected]

1-702-714-7001Extn: 9041

Pharmaceutical Sciences Journals

Ann Jose

[email protected]

1-702-714-7001Extn: 9007

Social & Political Science Journals

Steve Harry

[email protected]

1-702-714-7001Extn: 9042

 
© 2008- 2018 OMICS International - Open Access Publisher. Best viewed in Mozilla Firefox | Google Chrome | Above IE 7.0 version