Immunohistochemical and Molecular Detection of pH1N1 in NecropsiedPulmonary Tissues of Fatal Cases with Indeterminate Conventional Testing
- *Corresponding Author:
- Atanu Basu
Electron Microscopy and Histopathology Group
National Institute of Virology
Tel: 9120 26006217
E-mail: [email protected]
Received date: February 1, 2016; Accepted date: March 16, 2016; Published date: April 10, 2016
Citation: Shelke V, Potdar V, Chadha M, Kolhapure R, Prasad S, et al. (2016) Immunohistochemical and Molecular Detection of pH1N1 in Necropsied Pulmonary Tissues of Fatal Cases with Indeterminate Conventional Testing. J Foren Path 1:101. doi:10.4172/JFP.1000101
Copyright: © 2016 Shelke V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The rapid emergence of a novel influenza A/ H1N1 virus designated pH1N1 2009 caused one of the fastest pandemics of the twentieth century. The rapid development of an accurate detection test for this pandemic virus using reverse transcription-real time polymerase chain reaction (rRT-PCR) helped in timely diagnosis. In India this pandemic peaked between August to October 2009.The r-RT PCR for pH1N1 2009 was the main diagnostic test used on throat/nasopharyngeal swabs in all cases. While in majority of the cases this test provided reliable confirmation of the virus, it gave negative or indeterminate results in a subset of cases meeting the standard case definition for the pandemic infection and negative for seasonal flu. In the present study we examined 4 such fatal cases where microscopic pathology of the lung was consistent with viral bronchopneumonia for the presence of pH1N1 2009 using r-RT PCR on nucleic acid extracted from the paraffin sections that showed presence of viral antigens by immunohistochemistry. In all 4 cases pH1N1 sequences could be identified. These findings therefore emphasize the important role of microscopic pathology techniques in conjunction with molecular tools in the diagnostic confirmation of novel agents during a public health emergency.