Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley RatsFalana BA1,2, Ogundele OM3*, Duru FI2, Osinubi AA2 and Falode DT4
- *Corresponding Author:
- Ogundele OM
Department of Anatomy
College of Medicine and Health Sciences
Afe Babalola University, Ado-Ekiti, Nigeria
E-mail: [email protected]
Received date: February 28, 2013; Accepted date: April 02, 2013; Published date: April 05, 2013
Citation: Falana BA, Ogundele OM, Duru FI, Osinubi AA, Falode DT (2013) Immunohistochemical Characterization of Lymphocytic CD3/CD20 and Macrophage CD68 in the Germinal Epithelium of Pb and Se+Zn Treated Adult Sprague-Dawley Rats. J Cytol Histol 4:171. doi:10.4172/2157-7099.1000171
Copyright: © 2013 Falana BA, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Lead toxicity in the testes has been described to be capable of inducing cell death by apoptosis and necrosis. Such toxicity can be attenuated by selenium and zinc synergy treatment in trace amount. This study evaluates the role and distribution of macrophages/histocytes (CD68), B-Lymphocytes (CD20) and T-Lymphocytes (CD3) in the testes of lead, selenium and zinc treated rats. 60 F1 generation adult male Sprague-Dawley rats were divided into four groups of 15 animals each. Group 1 received normal saline, group 2: 100 mg/Kg of lead acetate, group 3: 100 mg/kg of lead acetate then 2.25 mg/ Kg each of Zinc (Chelated zinc) and Selenium (Sodium Selenium) and group 4: 2.25 mg/kg of zinc and selenium (Se+Zn). The duration of treatment was 56 days following which the animals were sacrificed on the 57th day and the testes harvested and fixed in Bouin’s fluid. CD3, CD20 and CD68 are distributed within the epithelium and the interstitium of the Pb treated testis, the expression level is influenced by the extent of the damage posed by Pb toxicity and not by the proliferative tendencies of Se+Zn treatment did protect the germinal epithelium and the macrophage/lymphocyte cell lines.