alexa Immunohistochemical Detection of Haemoglobin Subunit Epsilon (HBE) in the Developing Mouse Placenta | OMICS International| Abstract
ISSN: 2157-7099

Journal of Cytology & Histology
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  • Research Article   
  • J Cytol Histol 2019, Vol 10(3): 542

Immunohistochemical Detection of Haemoglobin Subunit Epsilon (HBE) in the Developing Mouse Placenta

Layla H Al-Kinani1,2,3, Flaminia Coiacetto1, Claire R Sharp1, Gabriele Rossi1 and Wayne K Greene2*
1School of Veterinary Medicine, Murdoch University, Perth W.A. 6150, Australia
2Discipline of Medical, Molecular and Forensic Sciences, Murdoch University, Perth WA 6150, Australia
3College of Veterinary Medicine, Baghdad University, Baghdad, Iraq
*Corresponding Author : Wayne K Greene, Discipline of Medical, Molecular and Forensic Sciences, Murdoch University, Perth WA 6150, Australia, Tel: (61-8) 9360 2545, Email: [email protected]

Received Date: Mar 24, 2019 / Accepted Date: Jun 11, 2019 / Published Date: Jun 18, 2019

Abstract

Introduction: Haemoglobin is a widely studied protein due to its important roles in physiology and pathology. Aberrant expression of haemoglobins, including primitive globins, have been reported in various sites and disease states and may have utility in some instances as diagnostic and/or prognostic markers. Despite this, robust detection of haemoglobin epsilon in the placenta during development by immunohistochemistry has not been well documented.
Aim: To evaluate a polyclonal antibody against human haemoglobin subunit epsilon (HBE) by immunohistochemistry during primitive erythropoiesis in the developing mouse placenta.
Methods and results: An immunohistochemistry protocol was developed using a commercially available antihuman haemoglobin subunit epsilon antibody on the mouse placenta at embryonic day 11.5. Strong and specific cytoplasmic staining was observed in primitive erythroid cells within the blood cell islands. By contrast, the placenta endothelium, mesothelium and mesoderm were all immunonegative for epsilon haemoglobin.
Conclusions: An immunohistochemistry protocol for the specific detection of epsilon haemoglobin was successfully developed using mouse placenta tissue. This assay has utility as a tool for the study of erythropoiesis during development and/or detecting the ectopic expression of epsilon globins in disease states such as cancer.

Keywords: DAB staining; Polyclonal antibody; Primitive haemopoiesis; Foetal erythrocytes

Citation: Al-Kinani LH, Coiacetto F, Sharp CR, Rossi G, Greene WK (2019) Immunohistochemical Detection of Haemoglobin Subunit Epsilon (HBE) in the Developing Mouse Placenta. J Cytol Histol 10: 542.

Copyright: © 2019 Al-Kinani LH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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